ピルビン酸キナーゼ 化学特性,用途語,生産方法
外観
タンパク質5～30mg/ml, 硫酸アンモニウム2.0～3.0mol/l, りん酸カリウム0.1mol/l, 懸濁液(pH7.0±0.5)
化学的特性
White powder
使用
Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency.
一般的な説明
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M
1, and M
2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M
1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M
2 is localized in the nucleus of the cells.
純化方法
These are purified by (NH4)2SO4 fractionation and gel filtration, ion-exchange and affinity chromatography. [Garcia-Olalla & Garrido-Pertierra Biochem J 241 573 1987.]
ピルビン酸キナーゼ 上流と下流の製品情報
原材料
準備製品