ddNTP set

ddNTP set 구조식 이미지
카스 번호:
상품명:
ddNTP set
동의어(영문):
ddNTP set;Dideoxynucleoside Triphosphate Set;Dideoxynucleotide triphosphate set
CBNumber:
CB01563049
분자식:
포뮬러 무게:
0
MOL 파일:
Mol file

ddNTP set 속성

저장 조건
-20°C (-15°C to -25°C)

안전

ddNTP set C화학적 특성, 용도, 생산

용도

2′,3′-Dideoxynucleoside triphosphates inhibit the chain elongation of a given primer catalyzed by the DNA polymerase (e.g., Klenow enzyme) and are therefore used for DNA sequencing according to Sanger (Sanger, F. et al. (1977) PNAS USA 74, 5463).
Sequencing is achieved by including in each reaction a dideoxynucleotide that acts as a chain terminator. Four reactions are set up, each containing the same template and primer, and a chain terminator specific for A, C, G, or T. Because only a small amount of the chain terminator is included, incorporation into the new DNA strand is a random event. Each reaction therefore generates a collection of fragments, and every DNA strand will end at the same type of base (A, C, G, or T).

일반 설명

The Dideoxynucleoside Triphosphate Set, Sequencing Grade, sodium salt solution, consists of dideoxynucleotides of very high purity (ddNTP HPLC, area% ≥98%; ddNDP HPLC, area% ≤1.5%), specifically manufactured and tested for sequencing reactions.
The set contains 4 individual vials of ddATP, ddCTP, ddGTP, and ddTTP, each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3).

ddNTP set 준비 용품 및 원자재

원자재

준비 용품


ddNTP set 공급 업체

글로벌( 4)공급 업체
공급자 전화 이메일 국가 제품 수 이점
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80

ddNTP set 관련 검색:

Copyright 2019 © ChemicalBook. All rights reserved