DPN I
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DPN I 속성
- 저장 조건
- -20°C
- 물리적 상태
- 완충된 글리세롤 수용액
안전
| 유엔번호(UN No.) | UN3373 - class 6.2 Biological substance, Category B | ||
|---|---|---|---|
| WGK 독일 | 3 |
DPN I C화학적 특성, 용도, 생산
용도
In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.일반 설명
IsoschizomersThe enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.
DPN I 준비 용품 및 원자재
원자재
준비 용품
DPN I 공급 업체
글로벌( 60)공급 업체
| 공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
|---|---|---|---|---|---|
| Career Henan Chemica Co | +86-0371-86658258 15093356674; |
laboratory@coreychem.com | China | 30255 | 58 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 821-50328103-801 18930552037 |
3bsc@sina.com | China | 15848 | 69 |
| Sigma-Aldrich | 021-61415566 800-8193336 |
orderCN@merckgroup.com | China | 51471 | 80 |
| Shanghai Kanglang Biotechnology Co., Ltd. | 021-61998208 18217752821 |
sales@klbio.cn | China | 9617 | 58 |
| Wuhan Chemstan Biotechnology Co., Ltd. | 15926423062 |
422450190@qq.com | China | 10132 | 58 |
| Shanghai Yuchun Biotechnology Co., Ltd | 021-021-52273538 17321241593 |
1503472510@qq.com | China | 5250 | 58 |
DPN I 관련 검색:
디스프로슘 트리(2,2,6,6-테트라메틸-3,5-헵탄디오네이트) 코발트(II) 아세틸아세토네이트 하이드레이트 아세틸아세톤산 구리 실버 아세틸아세토네이트 알루미늄 아세틸아세토네이트 아세틸아세토산 제2철 니코틴아미드아데닌 디뉴클레오티드
α-Nicotinamide adenine dinucleotide
Benzyl isocyanide
Tosylmethyl isocyanide
N-BUTYLISOCYANIDE
1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
PHENYLSELENOL
Tris(2,4-pentanedionato)chroMiuM(III)
TERT-BUTYL ISOCYANIDE
METHYL ISOCYANOACETATE
TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
SALCOMINE