TRANSCRIPTOR REVERSE TRANSCRIPTASE C화학적 특성, 용도, 생산
용도
Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler? Instruments) for the following applications:
Synthesis of first-strand cDNA for use in subsequent amplification reactions
RT-PCR of GC-rich RNA templates
Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
Retrieving and cloning the 5′ and 3′ termini of mRNA by RACE
Generation of cDNA libraries with large inserts
Dideoxy DNA sequencing
RNA sequencing
3′-end labeling of DNA fragments
Generation of single-stranded probes for genomic footprints
In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)
일반 설명
In retroviruses like the human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the core enzyme. HIV-1 RT is made of two subunits of 66?kDa and 51?kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family codes for reverse transcriptase (RT) enzyme.
Biochem/physiol Actions
Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into the double-stranded linear DNA that is integrated into host cell chromosomes.
