Bandeirea simplicifolia agglutinin, BS-I C화학적 특성, 용도, 생산
용도
General Western Blot Protocol:
Glycoprotein sample size: 500ng
Lectin Concentration: 0.1ug/ml
Load samples at 500 ng of glycoprotein per lane
Run 4-20% Bis-Tris SDS page gel
Transfer gel to a PVDF membrane
Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
Wash membrane 5 x 5 minutes with 25ml RIPA buffer
Detect using chemiluminescent substrate (CPS1-120)
일반 설명
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has A and B subunits, which recognizes αGalNAc and αGal-end groups respectively. Lectins are extracted from the seeds of the legume shrub Griffonia simplicifolia, which are carbohydrate binding proteins.
Biochem/physiol Actions
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Bandeirea simplicifolia agglutinin, BS-I 준비 용품 및 원자재
