닌히드린
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닌히드린 속성
- 녹는점
- 250 °C (dec.) (lit.)
- 끓는 점
- 230.35°C (rough estimate)
- 밀도
- 0.862
- 증기 밀도
- 6.16 (vs air)
- 굴절률
- 1.4209 (estimate)
- 인화점
- 13 °C
- 저장 조건
- Keep in dark place,Inert atmosphere,Room temperature
- 용해도
- 물(50mg/ml, 초음파 처리 포함), 에탄올(100mg/ml), 클로로포름(약간 용해됨), 에테르(약간 용해됨) 및 메탄올(100mg/ml)에 용해됩니다.
- 산도 계수 (pKa)
- 8.47±0.20(Predicted)
- 물리적 상태
- 분말/고체
- 색상
- 흰색에서 갈색을 띤 흰색, 결정
- Specific Gravity
- 0.788
- 냄새
- 냄새 없는
- pH 범위
- 4.6 - 5.0
- 수소이온지수(pH)
- 4.6-5.0 (10g/l, H2O, 20℃)
- 수용성
- 20°C에서 0.1-0.5g/100mL
- 감도
- Light Sensitive
- Merck
- 14,6554
- BRN
- 1910963
- 안정성
- 안정적인. 아민, 알칼리, 강염기와 호환되지 않습니다. 감광성.
- InChIKey
- FEMOMIGRRWSMCU-UHFFFAOYSA-N
- LogP
- 0.670
- CAS 데이터베이스
- 485-47-2(CAS DataBase Reference)
- NIST
- Ninhydrin(485-47-2)
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
위험품 표기 | Xn,T,F | ||
---|---|---|---|
위험 카페고리 넘버 | 22-36/37/38-39/23/24/25-20/21/22-11 | ||
안전지침서 | 26-28A-45-16-36 | ||
유엔번호(UN No.) | UN 1170 3/PG 2 | ||
WGK 독일 | 3 | ||
RTECS 번호 | NK5425000 | ||
F 고인화성물질 | 8 | ||
TSCA | Yes | ||
위험 등급 | 3 | ||
포장분류 | II | ||
HS 번호 | 29144000 | ||
독성 | LD50 orally in Rabbit: 600 mg/kg | ||
기존화학 물질 | KE-10839 |
닌히드린 C화학적 특성, 용도, 생산
개요
Ninhydrin is historically the most common reagent for processing fingerprints on porous substrates. It has been used as a fingerprint reagent since the 1950s but has been used as a cell stain since the early 1900s. Ninhydrin molecules form a complex with amino acids resulting in a purple product known as Ruhmann's purple. Purple fingerprints are easily visible on light-colored porous substrates but may be difficult to see on patterned or dark-colored substrates. DFO reacts with amino acids to produce a fluorescent product that is not readily seen with the naked eye. This makes it a useful reagent on dark or patterned porous items. DFO prints are viewed with an alternate light source or laser set to 530–570 nm (green light) through a red barrier filter. 1,2-indanedione is an emerging fingerprint reagent that results in visible pink fingerprints that are also fluorescent. The fluorescence is viewed under the same conditions as DFO.These chemicals can be used on their own or in sequence to develop latent prints on porous substrates. If they are used in sequence, they are used in the following order: indanedione, DFO, ninhydrin. If a reagent is used out of sequence, it will inhibit or limit the effectiveness of the other reagents. In this laboratory exercise you will develop fingerprints on a porous substrate using indanedione, DFO, and ninhydrin.
ninhydrin structure
화학적 성질
Ninhydrin, also known as Ninhydrin hydrate, is a white to pale yellow crystalline powder. It has a melting point of 241°C but decomposes at this temperature. When heated to 100°C or above, it changes color to red. Ninhydrin is soluble in water and ethanol and has slight solubility in ether and chloroform. When exposed to light or air, it gradually changes color and can clump when exposed to moisture. In solution with α-amino acids, or any α-amino substances can form a deep blue or even pink or red material, so it is often used as a detection of α-amino acids and peptides color developer.역사
Ninhydrin was discovered in 1910 by the German-English chemist Siegfried Ruhemann (1859–1943). In the same year, Ruhemann observed ninhydrin's reaction with amino acids. In 1954, Swedish investigators Oden and von Hofsten proposed that ninhydrin could be used to develop latent fingerprints.정의
ChEBI: Ninhydrin is a member of the class of indanones that is indane-1,3-dione bearing two additional hydroxy substituents at position 2. It has a role as a colour indicator and a human metabolite. It is a member of indanones, a beta-diketone, an aromatic ketone and a ketone hydrate.화학 반응
Ninhydrin reacts with primary and secondary amines (including amino acids, proteins, and peptides) to give a dark purple product known as Ruhemann's purple (RP) . As the eccrine component of a latent mark deposit contains amino acids, this reaction can be exploited as a means of developing fingermarks on porous surfaces such as paper and cardboard. The use of ninhydrin as a fingermark detection reagent was first proposed in 1954 by Odén and von Hofsten (1954). Since then, ninhydrin has become the most popular technique for fingermark detection on porous substrates.Eccrine glands secrete a range of different amino acids that may ultimately be present in a latent fingermark deposit (Hamilton 1965; Ramotowski 2001). Ninhydrin is a nonspecific amino acid reagent in that it reacts in the same manner with different amino acids. In this way, each amino acid present in the latent fingermark deposit will contribute to the developed fingermark image. Amino acids are stable compounds that, due to an affinity for cellulose, do not migrate to any significant extent through dry paper substrates. As a result, very old latent marks can be developed with ninhydrin (the development of 40-year-old marks has been recorded), and the revealed marks are normally of good quality. In addition, the amino acid composition of the eccrine secretion appears to remain relatively constant. Due to these qualities, the use of amino acid reagents (ninhydrin and ninhydrin analogs, including 1,8-diazafluoren-9-one [DFO]) constitutes an effective chemical technique for the development of latent fingermarks on paper surfaces.
일반 설명
White to light yellow crystals or powder. Becomes anhydrous with reddening at 257-266°F.공기와 물의 반응
Slightly soluble in water.반응 프로필
Ninhydrin hydrate is sensitive to prolonged exposure to light. Ninhydrin hydrate yields highly fluorescent ternary compounds with aldehydes and primary amines.위험도
Irritant.건강위험
Symptoms of exposure to this compound may include skin irritation and sensitization, and redness of the skin.ACUTE/CHRONIC HAZARDS: This compound causes irritation on contact. When heated to decomposition it emits acrid smoke and fumes.
화재위험
Flash point data for Ninhydrin hydrate are not available. Ninhydrin hydrate is probably combustible.Synthesis
The synthesis of Ninhydrin hydrate is as follows:A sealed-pressurised reaction vessel (5mL) equipped with a magnetic stirrer was charged with indan-1-one (1equiv), selenium dioxide (3.1equiv) and dioxane/water (3mL/0.3mL). It was then irradiated in a Biotage Initiator Microwave synthesizer 2.0 440W with microwave heating to 180°C with a maximum of 400W for 5min. Then, the vessel was rapidly forced-air cooled to room temperature. The mixture was transferred into a round bottom flask, and the vessel washed with acetone. Silica was added to prepare a solid deposit. The volatile solvents were then evaporated in vacuo before purification by flash chromatography (ethyl acetate/cyclohexane) to afford the corresponding ninhydrin.
Purification Methods
Crystallise ninhydrin from hot water (charcoal). Dry it under vacuum and store it in sealed brown containers. [Beilstein 7 IV 2786.]닌히드린 준비 용품 및 원자재
원자재
준비 용품
닌히드린 공급 업체
글로벌( 597)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Nanjing Triumpharm Co.,Ltd | +86-02586182925 +86-15105172550 |
info@triumpharm.com | China | 129 | 58 |
Hebei Mojin Biotechnology Co., Ltd | +8613288715578 |
sales@hbmojin.com | China | 12456 | 58 |
Hebei Guanlang Biotechnology Co,.LTD | +8619930503252 |
daisy@crovellbio.com | China | 5964 | 58 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21691 | 55 |
career henan chemical co | +86-0371-86658258 |
sales@coreychem.com | China | 29914 | 58 |
City Chemical LLC | 2039322489 |
sales@citychemical.com | United States | 168 | 58 |
Hebei Guanlang Biotechnology Co., Ltd. | +86-19930503282 |
alice@crovellbio.com | China | 8823 | 58 |
Xiamen AmoyChem Co., Ltd | +86-592-6051114 +8618959220845 |
sales@amoychem.com | China | 6387 | 58 |
Chongqing Chemdad Co., Ltd | +86-023-61398051 +8613650506873 |
sales@chemdad.com | China | 39916 | 58 |
Alchem Pharmtech,Inc. | 8485655694 |
sales@alchempharmtech.com | United States | 63711 | 58 |
닌히드린 관련 검색:
포수클로랄 p-토루엔 술폰산 일수화물 시트라진산 요오드포름 1,3-인단디온 닌히드린
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