Geranylgeranyl pyrophosphate

Geranylgeranyl pyrophosphate 속성

Geranylgeranyl pyrophosphate C화학적 특성, 용도, 생산

개요

Geranylgeranyl pyrophosphate (GGPP) is a potent endogenous regulator of Hmg2p degradation.This work was launched by the unexpected observation that GGPP addition directly to living yeast cultures caused high potency and specific stimulation of Hmg2p degradation. This effect of GGPP was not recapitulated by FPP,GGOH, or related isoprenoids. GGPP-caused Hmg2p degradation met all the criteria for the previously characterized endogenous signal.The action of added GGPP did not require production of endogenous sterol molecules, indicating that it did not act by causing the build-up of an endogenouspathway signal.

용도

Geranylgeranyl pyrophosphate ammonium salt has been used:
in the preparation of 2.5X reaction buffer for Rab GGTase assay
to add to the culture to further drive Hmg2 degradation
as a substrate to carryout in vitro enzymatic assays for the characterization of the prenyltransferases
to reverse the action of lovastatin and also used to examine the potential role of GGPP in the study to assess the effects of exposing the melanoma cell lines in the angiogenesis model as a co-culture to lovastatin?in vitro

Biochem/physiol Actions

Geranylgeranyl pyrophosphate (GGPP) is a major enzyme that participates in the secondary metabolism of chloroplast. GGPP synthase plays a key role in the biosynthesis of terpenoid. Protein geranylgeranylation, mediated by geranylgeranyl pyrophosphate participates in the development of the ventricular chamber. It also serves as a stage-specific signal to modulate the formation of cardiac cytoarchitecture the time of mid-gestation.

Purification Methods

Purify the pyrophosphate by countercurrent distribution between two phases of a butanol/isopropyl ether/ammonia/water mixture (15:5:1:19) (v/v), or by chromatography on DEAE-cellulose (linear gradient of 0.02M KCl in 1mM Tris buffer, pH 8.9). Alternatively purify it through a column of Dowex 1-x8 (formate form previously washed with MeOH) and eluted with a linear gradient of 0.053—0.43M ammonium formate in a total volume of 300mL of MeOH. The purity can be checked by TLC on Silica gel G on buffered plates (pH 6.5), eluted with CHCl3/MeOH/H2O (60:40:9) and developed with I2 vapour. Store it as a powder at 0o. [Altman et al. J Am Chem Soc 94 3257 1972.] Itis more stable as the di(tri-n-butylammonium)hydrogen phosphate salt which can be obtained from the acid by evaporation in a rotary evaporator below 32o [Upper & West J Biol Chem 242 3285 1967]. [Gregonis & Rilling Biochemistry 13 1538 1974, Gregonis & Rilling Biochem Biophys Res Commun 54 449 1973.]

Geranylgeranyl pyrophosphate 준비 용품 및 원자재

원자재

준비 용품

Geranylgeranyl pyrophosphate 공급 업체

공급자	전화	이메일	국가	제품 수	이점
3B Pharmachem (Wuhan) International Co.,Ltd.	821-50328103-801 18930552037	3bsc@sina.com	China	15848	69
Sichuan Wei Keqi Biological Technology Co., Ltd.	028-81700200 18116577057	3003855609@qq.com	China	7892	56
Wuxi Zhongkun Biochemical Technology Co., Ltd.	0510-85629785 18013409632	sales@reading-chemicals.com	China	15185	58
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Shanghai EFE Biological Technology Co., Ltd.	021-65675885 18964387627	info@efebio.com	China	9709	58
Shanghai Baoyisheng Biotechnology Co., LTD	15221020695	502277408@qq.com	China	466	58
Beijing OKA biological technology co., LTD	010-62971590 18548936886	3462612863@qq.com	China	6912	58
Shenzhen Polymeri Biochemical Technology Co., Ltd.	+86-400-002-6226 13028896684	sales@rrkchem.com	China	55717	58
Wuxi Helen Biotechnology Co., Ltd.,	0510-85629785 18013409632	sales@reading-chemicals.com	China	14092	58
Energy Chemical	021-58432009 400-005-6266	marketing@energy-chemical.com	China	44941	58

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