Duolink In Situ Red Starter Kit Mouse/Rabbit
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Duolink In Situ Red Starter Kit Mouse/Rabbit 속성
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
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| 예방조치문구: |
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Duolink In Situ Red Starter Kit Mouse/Rabbit C화학적 특성, 용도, 생산
용도
Duolink?proximity ligation assay(PLA?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Follow the Duolink? In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink? PLA Resource Center for information on how to run a Duolink? experiment, applications, troubleshooting, and more.
To perform a complete Duolink? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink? PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink? In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red? filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink? PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink? projects
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Duolink In Situ Red Starter Kit Mouse/Rabbit 준비 용품 및 원자재
원자재
준비 용품
Duolink In Situ Red Starter Kit Mouse/Rabbit 공급 업체
글로벌( 2)공급 업체
| 공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
|---|---|---|---|---|---|
| Sigma-Aldrich | 021-61415566 800-8193336 |
orderCN@merckgroup.com | China | 51471 | 80 |