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Cholesterol oxidase

Cholesterol oxidase 구조식 이미지
카스 번호:
9028-76-6
상품명:
Cholesterol oxidase
동의어(영문):
REC-COD;EC 1.1.3.6;EC: 1.1.3.6;Cholesterin oxidase;CHOLESTEROL OXIDASE;Oxidase, cholesterol;3-Hydroxysteroid oxidase;Cholecalciferol(VitaminD);CHOLESTEROL OXIDASE extrapure;CHOLESTEROL OXIDASE MICROBIAL
CBNumber:
CB3152524
분자식:
NULL
포뮬러 무게:
0
MOL 파일:
Mol file

Cholesterol oxidase 속성

저장 조건
2-8°C
용해도
50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)
물리적 상태
solution (slightly hazy)
색상
pale yellow

안전

안전지침서 24/25
WGK 독일 3
F 고인화성물질 10-21
HS 번호 35079090

Cholesterol oxidase C화학적 특성, 용도, 생산

화학적 성질

Cholesterol oxidase is a bacterial-specific flavoenzyme that catalyzes the oxidation and isomerisation of steroids containing a 3β hydroxyl group and a double bond at the 5–6 of the steroid ring system. The enzyme is a member of a large family of flavin-specific oxidoreductases and is found in two different forms: one where the flavin adenine dinucleotide (FAD) cofactor is covalently linked to the protein and one where the cofactor is non-covalently bound to the protein.
Cholesterol oxidases are secreted bacterial enzymes that catalyze the first step in the degradation of cholesterol. They are flavoenzymes containing the redox cofactor, flavin adenine dinucleotide (FAD). The enzyme catalyzes three chemical conversions. In the first step, called the reductive half-reaction, the 3β-hydroxyl group of the steroid ring system is oxidized to the corresponding ketone. Key to this conversion is the FAD cofactor, which becomes reduced in the process. In the second step the enzyme catalyzes isomerization of the double bond in the oxidized steroid ring system from the 5–6 position to 4–5 position, to give the final steroid product, cholest-4-en-3-one. In the final step of the enzyme, called the oxidative half-reaction, the reduced cofactor reacts with dioxygen and is thus reoxidized while O2 is reduced to H2O2.

용도

Cholesterol oxidases are part of a unique class of enzymes that are soluble proteins although they interact with highly insoluble substrates. The natural substrate, cholesterol, is an important membrane component exhibiting low solubility in the aqueous medium of the cell, hence the enzyme must interact with the lipid bilayer in order to for the substrate to partition out of the membrane and undergo oxidation at the enzyme active site. In this regard cholesterol oxidase is an interfacial enzyme as it binds transiently to the membrane surface during catalysis and can only access the substrate from the membrane phase. Other examples of interfacial enzymes include phospholipases (Berg et al., 2001; Gelb et al., 1995; Jain et al., 1995).
Cholesterol oxidase has a number of important commercial applications. Initial studies on the enzyme focused on its use for the determination of cholesterol in serum, HDL or LDL (see review, Smith and Brooks, 1976). Serum cholesterol levels are determined using a three-enzyme assay, including cholesterol esterase, cholesterol oxidase and peroxidase (Richmond, 1973; Allain et al., 1974; Richmond, 1976). More recently electrochemical biosensors with immobilized cholesterol oxidase have been employed to determine cholesterol levels in serum or food (Arya et al., 2007; Basu et al., 2007; Vidal et al., 2004). Determining the serum cholesterol levels is critical for the assessment of a variety of diseases including hypercholesterolemia, coronary heart disease and lipid disorders for estimating the risk of thrombosis and myocardial infarction.

Cholesterol oxidase 준비 용품 및 원자재

원자재

준비 용품


Cholesterol oxidase 공급 업체

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Shanghai Zheyan Biotech Co., Ltd.
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career henan chemical co
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Hubei New Desheng Materials Technology Co., Ltd. 18971041571
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3B Pharmachem (Wuhan) International Co.,Ltd. 86-21-50328103 * 801、802、803、804 Mobile:18930552037
86-21-50328109 3bsc@sina.com China 15881 69
Shanghai Hanhong Scientific Co.,Ltd. 021-54306202,021-54308259
+86-21-64545202 info@hanhonggroup.com China 43260 64
ShangHai LanJi Science and Technology Development Co., Ltd. 021-56373608/66107288/13391393747
021-66107288 liujun@lanji.cn/QQ493513614 China 298 62
ShangHai YuanYe Biotechnology Co., Ltd. 021-61312847 qq:2797782341
021-55068248 2797782341@qq.com China 4975 60
Nanjing Dulai Biotechnology Co., Ltd. 025-84699383 84699415 13382013214 13382013204
025-52453057 njduly@126.com China 900 55
Shanghai JONLN Reagent Co., Ltd. 400-0066-400;021-60496031
021-55660885 shjlsw@163.com China 10072 55

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