Fluorescein isothiocyanate
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Fluorescein isothiocyanate 속성
- 녹는점
- >360 °C(lit.)
- 저장 조건
- -20°C
- 용해도
- DMSO(약간 용해됨), 메탄올(약간 용해됨)
- 물리적 상태
- 가루
- 색상
- 갈색에서 진한 갈색까지
- Merck
- 14,4089
- BRN
- 1407295
- 안정성
- 수분에 민감한
- InChIKey
- WBSWSUYBEIMVMI-UHFFFAOYSA-N
- CAS 데이터베이스
- 27072-45-3(CAS DataBase Reference)
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
위험품 표기 | Xn | ||
---|---|---|---|
위험 카페고리 넘버 | 42/43-36/37/38-20/21/22-42 | ||
안전지침서 | 22-36-26-24/25-45-36/37 | ||
WGK 독일 | 3 | ||
F 고인화성물질 | 10 | ||
HS 번호 | 29321900 |
Fluorescein isothiocyanate C화학적 특성, 용도, 생산
개요
Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0
화학적 성질
Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.용도
Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.제조 방법
By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.정의
ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.Fluorescein isothiocyanate 준비 용품 및 원자재
원자재
5-Aminofluorescein
Sodium hydroxide-ethanol solution
레조시놀
4-Nitrophthalic anhydride
플루오레세인
4-Nitrofluorescein
준비 용품
Fluorescein isothiocyanate 공급 업체
글로벌( 205)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Zhengzhou Anhuida Chemical Co., Ltd | +8615903659408 |
admin@ahdchem.com | China | 298 | 58 |
Hebei Mojin Biotechnology Co., Ltd | +8613288715578 |
sales@hbmojin.com | China | 12452 | 58 |
Capot Chemical Co.,Ltd. | 571-85586718 +8613336195806 |
sales@capotchem.com | China | 29797 | 60 |
Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 |
info@dakenam.com | China | 14936 | 58 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21700 | 55 |
career henan chemical co | +86-0371-86658258 |
sales@coreychem.com | China | 29914 | 58 |
Hebei Guanlang Biotechnology Co., Ltd. | +86-19930503282 |
alice@crovellbio.com | China | 8823 | 58 |
Chongqing Chemdad Co., Ltd | +86-023-61398051 +8613650506873 |
sales@chemdad.com | China | 39916 | 58 |
CONIER CHEM AND PHARMA LIMITED | +8618523575427 |
sales@conier.com | China | 47465 | 58 |
SHANGHAI T&W PHARMACEUTICAL CO., LTD. | +86-021-61551413 +8618813727289 |
contact@trustwe.com | China | 5739 | 58 |
Fluorescein isothiocyanate 관련 검색:
싸이오사이안산 나트륨 싸이오사이안산암모늄(로단암몬) 페닐이소티오시아네이트 싸이오사이안산 칼륨 플루오레세인 크산텐 디벤질에테르 삼차부틸 아세트산 4-(1-히드록시에틸)아닐린 p- 토릴 이소티오시아네이트 4,4'-메틸렌비스페놀 알릴 아이소사이오사이아네이트
Erythromycin thiocyanate
Fluorescein isothiocyanate isomer I
3-S-Isothiuronium propyl sulfonate
BENZYL ISOTHIOCYANATE
Isocitric acid trisodium salt
Isochlorogenic acid C