PALMITOYL COENZYME A POTASSIUM SALT

PALMITOYL COENZYME A POTASSIUM SALT C화학적 특성, 용도, 생산

화학적 성질

White powder

Purification Methods

Possible impurities are palmitic acid, S-palmitoyl thioglycolic acid and S-palmitoyl glutathione. These are removed by placing ca 200mg in a centrifuge tube and extracting with Me2CO (20mL), followed by two successive extractions with Et2O (15mL) to remove S-palmitoyl thioglycolic acid and palmitic acid. The residue is dissolved in H2O (4 x 4 mL), adjusted to pH 5 and centrifuged to remove insoluble S-palmitoyl glutathione and other insoluble impurities. To the clear supernatant is added 5% HClO4 (6mL) whereby S-palmitoyl CoA precipitates. The precipitate is washed with 0.8% HClO4 (10mL) and finally with Me2CO (3x 5mL) and dried in vacuo. It is stable for at least one year in dry form at 0o in a desiccator (dark). Solutions are stable for several months at -15o. Its solubility in H2O is 4%. The adenine content is used as the basis of purity with max at 260 and 232nm ( 6.4 x 106 and 9.4 x 106 cm2/mol, respectively). Higher absorption at 232nm would indicate other thio ester impurities, e.g. S-palmitoyl glutathione, which absorb highly at this wavelength. Also the phosphate content should be determined, and acid phosphate can be titrated potentiometrically. [Seubert Biochemical Preparations 7 80 1960, Srer et al. Biochim Biophys Acta 33 31 1959, Kornberg & Pricer J Biol Chem 204 329 , 345 1953, Beilstein 26 III/IV 3665.]

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PALMITOYL COENZYME A POTASSIUM SALT 공급 업체

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Hubei Shishun Biotechnology Co. Ltd	027-59223025 15107168801	1718480011@qq.com	China	9994	58
Hubei Guangao Biotechnology Co., Ltd	027-027-59223056 18162699093	1208480011@qq.com	China	9951	58

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