MUNG BEAN NUCLEASE

MUNG BEAN NUCLEASE C화학적 특성, 용도, 생산

용도

The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.

일반 설명

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

Biochem/physiol Actions

Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.

MUNG BEAN NUCLEASE 준비 용품 및 원자재

원자재

준비 용품

MUNG BEAN NUCLEASE 공급 업체

공급자	전화	이메일	국가	제품 수	이점
career henan chemical co	+86-0371-86658258 15093356674;	factory@coreychem.com	China	29826	58
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
Henan Alfa Chemical Co., Ltd	+8618339805032	alfa4@alfachem.cn	China	12755	58
Hzymes Biotechnology Co. LTD	+86-02160879177 +86-13849862354	zhongtingting@hzymes.com	China	116	58
LEAPCHEM CO., LTD.	+86-852-30606658	market18@leapchem.com	China	43348	58
Aladdin Scientific	+1-833-552-7181	sales@aladdinsci.com	United States	57511	58
Jiangsu Jinpu Nuoan Biotechnology Co., Ltd	0512-82709698 15250590826	marketing@gproan.com.cn	China	9	58
Shanghai Macklin Biochemical Co.,Ltd.	15221275939 15221275939	shenlinxing@macklin.cn	China	15878	55
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Shanghai YuanYe Biotechnology Co., Ltd.	021-61312847; 18021002903	3008007409@qq.com	China	27322	60

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