REVERSE TRANSCRIPTASE

REVERSE TRANSCRIPTASE C화학적 특성, 용도, 생산

개요

Enzyme possessed by retroviruses that allows transcription of DNA from an RNA template. Such an enzyme has never been isolated from other sources, but speculation about its possible occurrence continues. Such an enzyme is widely used experimentally in genetic engineering to allow the formation of complementary DNA (cDNA) from purified RNA. The discovery of this enzyme faulted the widely held central dogma, which stated that the information flow in nature was always from DNA to RNA.

용도

Synthesizes a complementary DNA strand using single-stranded RNA or DNA in the presence of a primer

일반 설명

M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Purification Methods

This enzyme produces the complementary DNA from the RNA (as template). These are purified by solubilising the virus with non-ionic detergents. Lysed virions are adsorbed on DEAE-cellulose or DEAE-Sephadex columns, and the enzymes are eluted with a salt gradient, then chromatographed on a phosphocellulose column and fractions with enzyme activity are eluted in a salt gradient. They are also purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 1977, Smith Methods Enzymol 65 560 1980; see commercial catalogues for other transcriptases.]

REVERSE TRANSCRIPTASE 준비 용품 및 원자재

원자재

준비 용품

REVERSE TRANSCRIPTASE 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Shanghai Daken Advanced Materials Co.,Ltd	+86-371-66670886	info@dakenam.com	China	15928	58
career henan chemical co	+86-0371-86658258 15093356674;	factory@coreychem.com	China	29826	58
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
Hebei Duling International Trade Co. LTD	+8618032673083	sales05@hbduling.cn	China	15745	58
Nanjing Duly Biotech Co.,Ltd	+undefined18013301590	melody@njduly.com	China	2452	58
J & K SCIENTIFIC LTD.	010-82848833 400-666-7788	jkinfo@jkchemical.com	China	96815	76
BEST-REAGENT	400-1166-196 18981987031	cdhxsj@163.com	China	11726	57
Chengdu Ai Keda Chemical Technology Co., Ltd.	4008-755-333 18080918076	800078821@qq.com	China	9725	55
Shanghai Aladdin Bio-Chem Technology Co.,LTD	400-6206333 18521732826;	market@aladdin-e.com	China	25015	65
Nanjing Sunlida Biological Technology Co., Ltd.	025-57798810	sales@sunlidabio.com	China	3750	55

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