Calpain Substrate III, Fluorogenic - Calbiochem

Calpain Substrate III, Fluorogenic - Calbiochem C화학적 특성, 용도, 생산

일반 설명

A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (k_cat/K_mx10^-2 (M^-1s^-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC and α-spectrin cleavage site-based substrate. Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.

Biochem/physiol Actions

Product does not compete with ATP.

Calpain Substrate III, Fluorogenic - Calbiochem 준비 용품 및 원자재

원자재

준비 용품

Calpain Substrate III, Fluorogenic - Calbiochem 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80