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피브로넥틴

피브로넥틴
피브로넥틴 구조식 이미지
카스 번호:
86088-83-7
한글명:
피브로넥틴
동의어(한글):
피브로넥틴
상품명:
FIBRONECTIN
동의어(영문):
FN;HFN;cig;FINC;HFFN;FIBRONECTIN;Fibronectins;fibronectin 1;FIBRONECTIN, RAT;Einecs 289-149-2
CBNumber:
CB6306551
분자식:
NULL
포뮬러 무게:
0
MOL 파일:
Mol file

피브로넥틴 속성

저장 조건
-20°C
용해도
H2O: 1 mg/mL at 37 °C Store reconstituted solution in working aliquots at -20°C or lower.
물리적 상태
solution
수용성
Miscible with water.

안전

위험품 표기 B
WGK 독일 3
F 고인화성물질 10-21

피브로넥틴 C화학적 특성, 용도, 생산

용도

fibronectin (hydrolyzed) is a humectant and moisturizing agent for skin creams and lotions.

용도

fibronectin is a cosmetic ingredient described as a surface protective agent with a moisturizing and protective effect. Produced by many types of cells, fibronectin is a glycoprotein and an important component of the skin’s basal layer. Research suggests fibronectin plays a significant role in maintaining a healthy basal layer. Fibronectin’s function in the skin is to strengthen the attachments among collagen and elastin fibers, fibroblasts, and other cells in the dermis with connective tissues. Its presence may also be important to cell growth. There is some evidence that fibronectin may act as a cell regenerator by reversing abnormal cell formation and growth. It constitutes 1 to 3 percent of the total cellular protein of fibroblasts.

Purification Methods

This glycoprotein contains 5-12% of carbohydrate. It has been purified by glycine fractionation and DEAE-cellulose chromatography. This material is dissolved in 0.25M Tris-phosphate buffer pH 7.0, diluted to 20% and glycine added gradually till 2.1M when the temperature falls to below 15o. The precipitate contains mainly fibrinogen. The supernatant is discarded, and the precipitate is treated with an equal volume of H2O, cooled (to 0o) and precipitated by adding EtOH to 16% (v/v) at -4o. The precipitate contains some CI (Cold Insoluble) globulin, fibronectin and small quantities of other proteins. To remove these the precipitate is dissolved in 0.25M Tris-phosphate buffer (pH 7.0) ca 0.5% and purified by DEAE-cellulose chromatography after diluting the buffer to 0.05M buffer. [Morrison et al. J Am Chem Soc 70 3103 1948, Mosesson & Umfleet J Biol Chem 245 5728 1970, Mosesson & Amrani Blood 56 145 1980, Akiyama & Yamada Adv Enzymol 59 51 1987.]

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