CHONDROITINASE ABC

CHONDROITINASE ABC C화학적 특성, 용도, 생산

용도

Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.

Biochem/physiol Actions

Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.

CHONDROITINASE ABC 준비 용품 및 원자재

원자재

준비 용품

CHONDROITINASE ABC 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
Shanghai Acmec Biochemical Technology Co., Ltd.	+undefined18621343501	product@acmec-e.com	China	33349	58
Sinopharm Chemical Reagent Co,Ltd.	86-21-63210123	sj_scrc@sinopharm.com	China	9823	79
Shanghai Macklin Biochemical Co.,Ltd.	15221275939 15221275939	shenlinxing@macklin.cn	China	15878	55
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Beijing OKA biological technology co., LTD	010-62971590 18548936886	3462612863@qq.com	China	6912	58
Guangzhou Tomums Life Science Co., Ltd.	020-31155029 18902330969	sales@tomums.cn	China	4698	58
Shaanxi DIDU pharmaceutical and Chemical Co., Ltd	17691182729 18161915376	1046@dideu.com	China	10009	58
JiangSu Star Science Co.,Ltd	400-605-7200 17312445705	sales022@b2star.com	China	3764	58
Shanghai Yingxin Laboratory Equipment Co., Ltd.	021-021-59178156 17002132182	3146474380@qq.com	China	11303	58

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