Cephalins;L-A-PHOSPHATIDYLETHANOLAMINE TYPE III:*F ROM EGG YOL;1,2-Diacyl-sn-glycero-3-phosphoethanolamine, L-α-Cephalin;1,2-Diacyl-sn-glycero-3-phosphoethanolamine, 3-sn-Phosphatidylethanolamine, L-α-Cephalin;Kephalins;E COLI PE;Cephalinex;l-α-cephalin;Phospholipids cephalins;L-ALPHA-CEPHALIN E COLI
암을 일으킬 것으로 의심됨 (노출되어도 암을 일으키지 않는다는 결정적인 증거가 있는 노출경로가 있다면 노출경로 기재)
발암성 물질
구분 2
경고
P201, P202, P281, P308+P313, P405,P501
H372
장기간 또는 반복 노출되면 장기(또는, 영향을 받은 알려진 모든 장기를 명시)에 손상을 일으킴
특정 표적장기 독성 - 반복 노출
구분 1
위험
P260, P264, P270, P314, P501
H412
장기적 영향에 의해 수생생물에 유해함
수생 환경유해성 물질 - 만성
구분 3
P273, P501
예방조치문구:
P201
사용 전 취급 설명서를 확보하시오.
P273
환경으로 배출하지 마시오.
P302+P352
피부에 묻으면 다량의 물로 씻으시오.
P308+P313
노출 또는 접촉이 우려되면 의학적인 조치· 조언를 구하시오.
세팔린 C화학적 특성, 용도, 생산
용도
L-α-Phosphatidylethanolamine from egg yolk has been used:
as a component of lipid mixture for the synthesis of giant unilamellar vesicles (GUVs)
in ceramide phosphoethanolamine (CPE) synthase assay using crude sphingomyelin synthase-related protein from Hela cells
for the standard curve generation for the quantification of lipids in plantaris muscle homogenates from mice
일반 설명
L-α-Phosphatidylethanolamine from egg yolk has high unsaturated fatty acid content and elicits radical-scavenging activity. L-α-Phosphatidylethanolamine (PE) is a membrane phospholipid and is interconverted to phosphotidylserine in the presence of phosphatidylserine synthase 2. PE is essential for maintaining the structural integrity of membranes. It is the second most abundant phospholipid in animals, plants and yeast and the major lipid in bacteria. It is synthesized in mitochondria in mammals.
효소 저해제
This hygroscopic phospholipid, also called cephalin and 1,2-diacyl-snglycero- 3-phosphoethanolamine, is an important constituent of biomembranes and lipoproteins. It is the major structural phospholipid in brain tissue. The physical properties depend on the nature of the acyl groups and on the presence of other lipids. Freshly prepared phosphatidylethanolamines are white solids that turn yellow and brown on exposure to air. They typically sinter between 80 and 90°C and melt in the neighborhood of 175°C. Solutions of phosphatidylethanolamine slowly decompose at room temperature (roughly 0.3-0.5% per day); hence, solutions should always be freshly prepared and kept cold prior to use. It is labile in alkaline conditions. See also specific compound Target(s): b- N-acetylglucosaminyl-glycopeptide b-1,4-galactosyltransferase; Nacetyllactosamine synthase; acylglycerol kinase, or monoacylglycerol kinase; cholesterol monooxygenase, side-chain-cleaving, or CYP11A1; chymase; cytidylate cyclase; dolichyldiphosphatase; dolichyl-phosphatase; dopamine b-monooxygenase; ethanolaminephosphotransferase; glutamate dehydrogenase; γ-glutamyl transpeptidase, mildly inhibited; glycerol-3-phosphate Oacyltransferase; glycerone-phosphate O-acyltransferase, or dihydroxyacetione-phosphate O-acyltransferase; hormone-sensitive lipase; 3-hydroxybutyrate dehydrogenase; indole-3-acetate b-glucosyltransferase; phosphatidate cytidylyltransferase; 1- phosphatidylinositol 4-kinase; phosphoinositide phospholipase C; phospholipase D, phosphatidylcholine-specific enzyme; sphingomyelin phosphodiesterase; steryl-b-glucosidase; UDP-Nacetylglucosamine: dolichyl-phosphate N-acetylglucosaminephosphotransferase.
Purification Methods
Purify the cephalin by dissolving it in EtOH, adding Pb(OAc)2.3H3O (30g in 100mL H2O) until excess Pb2+ is present. Filter off the solid. Pass CO2 gas through the solution until precipitation of PbCO3 ceases. Filter the solid off and evaporate (while bubbling CO2) under vacuum. An equal volume of H2O is added to the residual oil and extracted with hexane. The hexane extract is washed with H2O until the aqueous phase is free from Pb [test with dithizone (2 mg in 100 mL CCl4; Feigel Spot Tests Vol I, Elsevier p. 10 1954)]. The hexane is dried (Na2SO4), filtered and evaporated to give a yellow waxy solid which should be dried to constant weight in vacuo. It is practically insoluble in H2O and Me2CO, but freely soluble in CHCl3 (5%) and Et2O, and slightly soluble in EtOH. [Schofield & Dutton Biochemical Preparations 5 5 1957.]
