Duolink In Situ Detection Reagents Red

Duolink In Situ Detection Reagents Red C화학적 특성, 용도, 생산

용도

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink^? In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink^? PLA Resource Center for information on how to run a Duolink^? experiment, applications, troubleshooting, and more.

To perform a complete Duolink^? PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^? PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
Red fluorescence detection reagents are often used with Texas Red^? filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^? in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^? projects

View full Duolink^? product list

Duolink In Situ Detection Reagents Red 준비 용품 및 원자재

원자재

준비 용품

Duolink In Situ Detection Reagents Red 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
JiangSu Star Science Co.,Ltd	400-605-7200 17312445705	sales022@b2star.com	China	3764	58