DIG Gel Shift Kit, 2nd generation has been used for nonradioactive detection of sequence-specific DNA binding proteins. The DIG Gel Shift Kit is used to prepare nonradioactive, 3′-end labeled oligonucleotide probes to detect DNA-protein complexes in a "gel mobility shift assay".
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DIG Gel Shift Kit, 2nd generation contains reagents for nonradioactive 3′-end labeling of oligonucleotides, to be used in a "gel mobility shift" assay. It also contains offers, electrophoresis reagents and an enzyme-labeled antibody to facilitate the detection of DNA-protein complexes. DIG Gel Shift Kit employs recombinant terminal transferase and digoxigenin (DIG)-11- dideoxyuridine triphosphate (ddUTP) makes the labeling reaction very flexible. It can be used to label the 3′ ends of any oligonucleotide (whether it has a 5′- overhanging end, a 3′-overhanging end, or blunt ends). Both single- and double-stranded DNA can be labeled.
