TTH DNA POLYMERASE

TTH DNA POLYMERASE C화학적 특성, 용도, 생산

용도

Thermus thermophilus (Tth) DNA Polymerase might be used:

• to amplify DNA fragments by polymerase chain reaction (PCR) due to its resistance towards prolonged incubations at high temperatures (95 °C)
• to label DNA fragments with either radiolabeled nucleotides, digoxigenin, or biotin, since this enzyme accepts modified deoxyribonucleotides as substrates
• to efficiently transcribe RNA targets into cDNA due to its intrinsic Mn-dependent reverse transcriptase (RT) activity
• for real time PCR

일반 설명

Tth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly processive 5′-3′ DNA polymerase, and lacks 3′-5′ exonuclease activity. The enzyme exhibits its highest activity at a pH of approximately 9 (adjusted at +25°C), and temperatures around +75°C. It is resistant to prolonged incubations at high temperatures (+95°C).

Biochem/physiol Actions

Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5′-3′ DNA polymerase and lacks 3′-5′ exonuclease activity (proof reading). It was found to possess a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions – much higher than that demonstrated for Escherichia coli DNA polymerase I and Taq DNA Polymerase. The RT activity is not associated with RNase H activity. The elevated temperatures of Tth DNA Polymerase (optimum +55°C to +70°C, maximum +95°C) activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg2+-ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA. Tth DNA Polymerase is used for RT-PCR amplification of RNA up to 1kb.

TTH DNA POLYMERASE 준비 용품 및 원자재

원자재

준비 용품

TTH DNA POLYMERASE 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Yinjia (Shanghai) Bio-pharmaceutical Technology Co. , Ltd.	021-52992719 13512169707	product@yinjibio.com	China	29	58
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80
Beijing Solarbio Science & Tecnology Co., Ltd.	010-50973130 4009686088	3193328036@qq.com	China	29797	68
Wuhan Peptide Core Biotechnology Co., Ltd	027-65317797 13667154760	1096814016@qq.com	China	9907	58

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