니트로 BT
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니트로 BT 속성
- 녹는점
- 200°C
- 밀도
- 1.5521 (rough estimate)
- 굴절률
- 1.7350 (estimate)
- 저장 조건
- 2-8°C
- 용해도
- 메탄올: 50mg/mL, 투명하고 매우 강한 노란색
- 물리적 상태
- 분말/고체
- 색상
- 노란색
- 수용성
- 물, 에탄올, 메탄올에 용해됩니다.
- 감도
- Light Sensitive
- 최대 파장(λmax)
- 256 nm
- BRN
- 4115923
- 안정성
- 안정적이지만 차갑게 보관하세요.
- 생물학 분야의 응용
- Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
위험품 표기 | Xn,Xi | ||
---|---|---|---|
위험 카페고리 넘버 | 22-68/20/21/22-20/21/22-36-36/37/38-41 | ||
안전지침서 | 36-45-36/37-26-39 | ||
WGK 독일 | 3 | ||
RTECS 번호 | XF8045000 | ||
F 고인화성물질 | 8-10-23 | ||
TSCA | Yes | ||
HS 번호 | 32041900 | ||
독성 | LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990 | ||
기존화학 물질 | KE-11045 |
니트로 BT C화학적 특성, 용도, 생산
개요
Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.화학적 성질
NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.용도
Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.주요 응용
Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.
정의
ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).일반 설명
Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.위험도
Moderately toxic by ingestion.Biochem/physiol Actions
Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.Safety Profile
Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.니트로 BT 준비 용품 및 원자재
원자재
준비 용품
니트로 BT 공급 업체
글로벌( 443)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Suzhou Laing Biotechnology Co.,Ltd | +86-13004516015 +86-18149392822 |
christina@laingbiotec.com | China | 778 | 58 |
Hebei Mojin Biotechnology Co., Ltd | +86 13288715578 +8613288715578 |
sales@hbmojin.com | China | 12446 | 58 |
Shandong Juchuang Chemical Co., LTD | +undefined15030412209 |
admin@juchuangchem.com | China | 387 | 58 |
HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD | +8615383190639 |
admin@86-ss.com | China | 1000 | 58 |
Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 |
info@dakenam.com | China | 18628 | 58 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21667 | 55 |
Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418679 +8618949832763 |
info@tnjchem.com | China | 2989 | 55 |
career henan chemical co | +86-0371-86658258 +8613203830695 |
sales@coreychem.com | China | 29888 | 58 |
HaBo Hong Kong Co., Limited. | +86-25-18512596065 |
info@habotech.com | CHINA | 933 | 58 |
Accela ChemBio Inc. | +1-858-6993322 |
info@accelachem.com | United States | 19536 | 58 |