장기(또는, 영향을 받은 알려진 모든 장기를 명시)에 손상을 일으킬 수 있음(노출되어도 특정 표적장기 독성을 일으키지 않는다는 결정적인 노출경로가 있다면 노출경로를 기재)
특정 표적장기 독성 - 2회 노출
구분 2
경고
P260, P264, P270, P309+P311, P405,P501
예방조치문구:
P260
분진·흄·가스·미스트·증기·...·스프레이를 흡입하지 마시오.
P264
취급 후에는 손을 철저히 씻으시오.
P264
취급 후에는 손을 철저히 씻으시오.
P270
이 제품을 사용할 때에는 먹거나, 마시거나 흡연하지 마시오.
P405
밀봉하여 저장하시오.
P501
...에 내용물 / 용기를 폐기 하시오.
KAPA2G Fast Genotyping Mix C화학적 특성, 용도, 생산
용도
KAPA2G Fast Genotyping Mix:
has been used for the extraction and amplification from mouse tail, ear, and toe tissue
has been used for the extraction and amplification of DNA from other animal tissue and hyridomas
is suitable for use in polymerase chain reaction (PCR)
일반 설명
KAPA2G Fast Genotyping Mix (2X) is a ready-to-use master mix containing all components for fast polymerase chain reaction (PCR), except primers and template. The ReadyMix? also contains two inert dyes, allowing for the analysis of PCR reaction products by agarose gel electrophoresis without the need to add a DNA loading solution. The KAPA Express Extract system contains a thermostable protease, designed for rapid sample lysis. The KAPA Express Extract, a novel thermostable protease and buffer system allows the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols.
Biochem/physiol Actions
DNA extracted with KAPA Express Extract is amplified with KAPA2G Fast Genotyping Mix with dye. DNA fragments generated with KAPA2G Fast Genotyping Mix are 3′-dA-tailed and may be cloned into TA cloning vectors, or used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is like that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. Lysis is performed in a thermocycler, heating block or water bath, after which the sample is centrifuged to recover the DNA containing supernatant. The supernatant is then diluted 10-fold before use as a template in PCR. Unlike existing protocols that rely on Proteinase K digestion, extractions using KAPA Express Extract are conveniently performed in a single tube, without the need for hazardous chemicals, or multiple wash steps. This greatly reduces the risk of sample loss and contamination. The process yields enough template for multiple assays and is easily scaled to handle samples in 96-well format. Each extraction yields enough template for at least 100 genotyping PCR reactions. Extracts (either diluted or undiluted) are stable at -15°C to -25°C for at least 6 months.
