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4-몰포린-1-일에틸설폰산 나트륨 C화학적 특성, 용도, 생산
화학적 성질
White powder
용도
MES sodium salt is a buffering agent used in biological and biochemical research including plant cell culture. It has been used: To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA. To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization.
제조 방법
The amine starting material, aqueous sodium 2-hydroxyethanesulfonate, and aqueous 50 percent sodium hydroxide were charged to the autoclave for reaction. After purification, MES sodium salt can be obtained.
정의
ChEBI: Sodium 2-(N-morpholino)ethanesulfonate is the organic sodium salt that is the sodium salt of 2-(N-morpholino)ethanesulfonic acid. It contains a 2-(N-morpholino)ethanesulfonate.
일반 설명
MES is one of the so-called "Good" buffers developed for biological applications. This product is BioPerformance certified (suitable for electrophoresis, tissue culture, and molecular biology applications. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
