DIG-HIGH PRIME C화학적 특성, 용도, 생산
용도
DIG-High Prime-labeled DNA probes has been used in a variety of hybridization techniques :
- in Southern blots
- in northern blots
- in dot/slot blots
- for screening of gene libraries
- in In situ hybridizations
Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1μg total human DNA. The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots.
일반 설명
DIG-High Prime has enzyme and nucleotide mixture for rapid random-primed labeling of DNA with Digoxigenin-11-dUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
DIG-HIGH PRIME 준비 용품 및 원자재
원자재
준비 용품
