Triphosphopyridine nucleotide

Triphosphopyridine nucleotide 속성

Triphosphopyridine nucleotide C화학적 특성, 용도, 생산

화학적 성질

Triphosphopyridine nucleotide is white or off-white powder, it is easy to absorb moisture and deliquescence. pKa{1}=3.9; pKa{2}=6.1. It is soluble in water, methanol, insoluble in ethanol, insoluble in ether and ethyl acetate.

용도

β-Nicotinamide adenine dinucleotide phosphate hydrate is suitable for use in:
the measurement of Glucose-6-phosphate dehydrogenase activity
the Cytochrome P450 3A4 assay as a part of NADPH-regenerating system
the Cytochrome P450 2D6 assay as a part of NADPH-regenerating system
the determination of Glucose-6-phosphate content

정의

The oxidized form of nicotinamide adenine dinucleotide phosphate (NADP) that receives electrons from photosystem I during photosynthesis. It exists as an anion under normal physiologic conditions.

Synthesis

Triphosphopyridine nucleotide is prepared by the reaction of NADPH. It is synthesised mainly by the interaction of both NfrA1 enzyme and a Bacillus subtilis under the conditions of bacterial luciferase. Reaction conditions were as follows: with hydrogenchloride; NfrA1 enzyme; nitrofurazone; 2-amino-2-hydroxymethyl-1,3-propanediol In water at 23℃; pH=7.0; Enzyme kinetics; Further Variations; Reagents; Oxidation.

Purification Methods

Purify NMN by passage through a column of Dowex-1 (Clform) and washing with H2O until no absorbance is observed at 260 nm. The tubes containing NMN are pooled, adjusted to pH 5.5-6 and evaporated in vacuo to a small volume. This is adjusted to pH 3 with dilute HNO3 in an ice-bath and treated with 20volumes of Me2CO at 0-5o. The heavy white precipitate is collected by centrifugation at 0o. It is best stored wet and frozen or it can be dried to give a gummy residue. It has max 266nm ( 4,600) and min 249nm ( 3600) at pH 7.0 (i.e. no absorption at 340nm). It can be estimated by reaction with CNor hydrosulfite which form the 4-adducts (equivalent to NADH) which have UV max 340nm ( 6,200). Thus after reaction, an OD340 of one is obtained from a 0.1612mM solution in a 1cm path cuvette. [Plaut & Plaut Biochemical Preparations 5 56 1957, Maplan & Stolzenbach Methods Enzymol 3 899 1957, Kaplan et al. J Am Chem Soc 77 815 1955, Beilstein 22/2 V 168.]

Triphosphopyridine nucleotide 준비 용품 및 원자재

원자재

준비 용품

Triphosphopyridine nucleotide 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Maintain Biotech Co., Ltd.	+86-75589664337 +86-15112661142	cami@maintain-bio.com	China	47	58
Hubei Aumei New Material Co., Ltd.	+86-027-87366298 +8615927270571	tianyi@skbiology.cn	China	294	58
Wuhan Aoliqisi New Material Technology Co., Ltd.	+86-13545906766; +8613545906766	sales@whaop.com	China	265	58
Wuhan Golt Biotech Co., Ltd.	+8615389281203	maria@goltbiotech.com	China	980	58
SyncoZymes (Shanghai) Co., Ltd.,	+86-021-68187180-811 +86-13681683526	lchen@syncozymes.com	China	188	58
Shaanxi LonierHerb Bio Technology Co Ltd	+86-86-+86-86-029-87551862 +8617702909819	sales006@ingredients-lonier.com	China	2596	58
Hebei Mojin Biotechnology Co., Ltd	+8613288715578	sales@hbmojin.com	China	12453	58
Wuhan Haorong Biotechnology Co.,ltd	+8618565342920	sales@chembj.net	China	269	58
Shaanxi Haibo Biotechnology Co., Ltd	+undefined18602966907	qinhe02@xaltbio.com	China	1000	58
Nantong Guangyuan Chemicl Co,Ltd	+undefined17712220823	admin@guyunchem.com	China	616	58

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