AXIS Axon Isolation Device, 150 μm, Plasma Bonded

AXIS Axon Isolation Device, 150 μm, Plasma Bonded C화학적 특성, 용도, 생산

용도

Research Category
Neuroscience

일반 설명

These Plasma Bonded AXIS products are AXIS devices that have been permanently sealed to glass bottomed tissue culture dishes using plasma gas exposure. This format essentially eliminates issues with leakage or cell penetration under the device and should facilitate researchers in their live or fixed cell confocal imaging. These plasma bonded devices are currently being offered in three different formats to provide test flexibility during investigation.

AX15005PBC consists of 5 units of AX150, an axon isolation device consisting of four wells, two channels, and a set of microgrooves. Two of the wells are interconnected by a channel on each side of the device. The microgrooves are located in the area between the two channels and their length is 150μm. Each microgroove is approximately 5μm in height by 10μm in width and are designed to allow neurite passage to traverse through while preventing cell bodies from flowing across the device. There are roughly 120 microgrooves in each AX150 device.

The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009) and will likely work with most neuronal cell type that can be successfully grown in tissue culture. Because the AXIS devices are made from an inert and non-toxic polymer, cultures grown within AXIS devices can be maintained for weeks at a time. (Chun et al., 1992; Shibayama et al., 1991). In addition, because the polymer used to fabricate the axon isolation devices is optically clear, cells cultured within the device can be imaged with high resolution microscopy, including live cell imaging, confocal, and differential interference microscopy (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009).

The microfluidic design of the AXIS devices is highly conducive to generation of hydrostatic pressure, which can be selectively used to isolate a solution, as well as chemical targets of interest, on a designated side of the device (Park et al., 2006). In addition, immunocytochemistry (IHC) can also be performed within an AXIS Axon Isolation device and a protocol to do so is provided in the manual.

AXIS Axon Isolation Device, 150 μm, Plasma Bonded 준비 용품 및 원자재

원자재

준비 용품

AXIS Axon Isolation Device, 150 μm, Plasma Bonded 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80