| Company Name: |
Merck KGaA
|
| Tel: |
21-20338288 |
| Email: |
sigmaname@qq.com |
| Products Intro: |
Cas:150347-59-4
ProductName:5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
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- Cyanine3.5 NHS ester
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- $0.00 / 25mg
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2025-06-07
- CAS:2231670-86-1
- Min. Order: 25mg
- Purity: >99.00%
- Supply Ability: 25mg
- Cyanine5 NHS ester
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- $0.00 / 25mg
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2025-06-07
- CAS:1032678-42-4
- Min. Order: 25mg
- Purity: >99.00%
- Supply Ability: 25mg
- Cyanine7 NHS ester
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- $0.00 / 25mg
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2025-06-07
- CAS:2408482-09-5
- Min. Order: 25mg
- Purity: >95.00%
- Supply Ability: 25mg
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| | Nhs Ester Basic information |
| | Nhs Ester Chemical Properties |
| Melting point | 152-154 °C(lit.) | | storage temp. | -20°C | | solubility | DMF: soluble | | pka | 6.45(at 25℃) | | form | Solid | | color | Off-white to yellow | | PH Range | Weak green ' uorescence (6.0) to strong green ' uorescence (7.2) | | Appearance | white solid | | λmax | <300nm | | Major Application | Diagnosis of cancer, restricting HIV replication, antiinflammatory agent, detecting bacteria, cell proliferation assays | | InChIKey | QSWYNOQSGMUDQZ-UHFFFAOYSA-N | | SMILES | C12(OC(=O)C3=CC(C(=O)ON4C(=O)CCC4=O)=CC=C13)C1C=CC(OC(=O)C)=CC=1OC1C=C(OC(=O)C)C=CC2=1 |
| WGK Germany | 3 | | F | 8-10-21 | | HS Code | 32129000 |
| | Nhs Ester Usage And Synthesis |
| Description | (5,6)-FAM diacetate NHS ester is a cell-permeable amine-reactive label for biomolecules.
This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore with emission in the green spectrum range (absorption maximum at ~492 nm, emission maximum at ~517 nm). Due to this characteristic, this product is used for tracking cells and flow cytometric evaluations and studying intracellular esterase. | | Chemical Properties | 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is Off-white to light yellow powder | | Uses | 5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is a Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell. | | Uses | A 5(6)-Carboxyfluorescein (C178215) derivative used as a flurorescent probe. 5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester is used to monitor lymphocyte proliferation by covalently labelling long-lived intracellular molecules with fluorescent dye. | | Uses | Amine-reactive succinimidyl ester can potentially be used for long-term pH studies of live cells, producing a conjugate with the pH-sensitive properties of carboxyfluorescein. | | General Description | 5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation. | | Biochem/physiol Actions | 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity. | | Abs/Em Maxima | 492/517 nm |
| | Nhs Ester Preparation Products And Raw materials |
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