尼罗红
中文名称 | 尼罗红 |
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中文同义词 | 尼罗红 500MG;9-(二乙胺基)-5H-苯并[A]吩恶嗪-5-酮;尼罗红(尼罗丝、9-(二乙胺基)-5H-苯并[A]吩恶嗪-5-酮);NILE RED 尼罗红;尼罗红;尼罗红/尼罗丝;尼罗红(>95%,BS);尼罗丝 |
英文名称 | Nile Red |
英文同义词 | NILE RED;9-(diethylamino)-5h-benzo[a]phenoxazin-5-on;nile;9-(diethylamino)benzo[a]phenoxazin-5(5H)-one;FLUORESCENCE QY-STANDARD E CERTIFIED;9-(Diethylamino)benzo[a]phenoxazin-5(5H)-on;Nile Red, pure, 99%;9-Diethylamino-benzo[a]phenoxazin-5-one |
CAS号 | 7385-67-3 |
分子式 | C20H18N2O2 |
分子量 | 318.37 |
EINECS号 | 230-966-0 |
相关类别 | 其他生化试剂;生化试剂;荧光探针,标记,颗粒和染色;色素类;化工原料;生化试剂-色素类;通用生化试剂-生物染色剂与化学指示剂;有机化工原料;marker;Electroluminescence;Functional Materials |
Mol文件 | 7385-67-3.mol |
结构式 | ![]() |
尼罗红 性质
熔点 | 203-205 °C(lit.) |
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沸点 | 457.53°C (rough estimate) |
密度 | 1.1871 (rough estimate) |
折射率 | 1.5700 (estimate) |
储存条件 | 2-8°C |
溶解度 | 在甲醇中可溶1mg/mL |
酸度系数(pKa) | 4.08±0.20(Predicted) |
形态 | 粉末 |
颜色 | 深红色至栗色 |
最大波长(λmax) | 553 nm |
BRN | 279110 |
稳定性 | 稳定的。与强氧化剂不相容。 |
InChI | InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
InChIKey | VOFUROIFQGPCGE-UHFFFAOYSA-N |
SMILES | C12=CC=CC=C1C(=O)C=C1C2=NC2=C(C=C(N(CC)CC)C=C2)O1 |
CAS 数据库 | 7385-67-3(CAS DataBase Reference) |
EPA化学物质信息 | 5H-Benzo[a]phenoxazin-5-one, 9-(diethylamino)- (7385-67-3) |
Target | Value |
lipid droplet
() |
Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.
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