Company Name: |
3B Pharmachem (Wuhan) International Co.,Ltd.
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Tel: |
821-50328103-801 18930552037 |
Email: |
3bsc@sina.com |
Products Intro: |
Product Name:ANTI-MOUSE IGG (FAB SPECIFIC)-FITC Purity:99% HPLC Package:1Mg ; 5Mg;10Mg ;100Mg;250Mg ;500Mg ;1g;2.5g ;5g ;10g
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Company Name: |
Sigma-Aldrich
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Tel: |
021-61415566 800-8193336 |
Email: |
orderCN@merckgroup.com |
Products Intro: |
Product Name:Anti-Mouse IgG (Fab specific)FITC Purity:affinity isolated antibody, buffered aqueous solution Package:0.5mL, 1mL, 2mL Remarks:F5262
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Company Name: |
Riedel-de Haen AG
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Tel: |
800 558-9160 |
Email: |
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Products Intro: |
Remarks:affinity isolated antibody, buffered aqueous solution
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Company Name: |
SIGMA-RBI
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Tel: |
800 736 3690 (Orders) |
Email: |
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Products Intro: |
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| ANTI-MOUSE IGG (FAB SPECIFIC)-FITC Basic information |
| ANTI-MOUSE IGG (FAB SPECIFIC)-FITC Chemical Properties |
storage temp. | −20°C | form | buffered aqueous solution |
| ANTI-MOUSE IGG (FAB SPECIFIC)-FITC Usage And Synthesis |
Uses | Anti-Mouse IgG (Fab specific) F(ab′)2 fragment?FITC antibody produced in goat has been used in:
- flow cytometry for fluorescence-activated cell sorting (FACS) analysis
- immunofluorescence and confocal microscopy
- immunofluorescent HSP72 detection/immunocytochemistry experiments
| Uses | Anti-Mouse IgG (Fab Specific) FITC Conjugate antibody is used in traditional method of immunocytochemical assessment of ZAP-70 expression. It can also be used in immunocytochemical observation of the SCNT embryos. | Uses | Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:128. It may be used for imunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:150. In an agar diffusion assay the conjugate produces a precipitation arc at a dilution of 1:32 versus a 1:80 dilution of normal mouse serum. The antibody was used at a dilution range of 1:50 to 1:300 for immunofluorescence in various studies. | General Description | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG to ensure minimal cross reactivity. | Biochem/physiol Actions | IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions. IgG4 is associated with asymptomatic infection. Digestion of IgG by papain results in generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies. |
| ANTI-MOUSE IGG (FAB SPECIFIC)-FITC Preparation Products And Raw materials |
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