| Company Name: |
Sigma-Aldrich
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| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA
Purity:peroxidase substrate Package:100mL, 1L, 1L Remarks:T0440
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| Company Name: |
Otto Chemie Pvt. Ltd.
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| Tel: |
+91 9820041841 |
| Email: |
info@ottokemi.com |
| Products Intro: |
Product Name:3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA
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| Company Name: |
Creative Enzymes
|
| Tel: |
1-516-855-7709 |
| Email: |
info@creative-enzymes.com |
| Products Intro: |
Product Name:3,3',5,5'-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA
|
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| | 3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Basic information |
| | 3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Chemical Properties |
| | 3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Usage And Synthesis |
| Uses | TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.
How to use our TMB substrate:
- Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
- Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
- Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
- 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System is not recommended for membrane applications. Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
Note:TMB substrate is light-sensitive and should be protected from direct sunlight or UV sources. | | General Description | TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP. |
| | 3,3μ,5,5μ-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA Preparation Products And Raw materials |
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