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Product Name:Trehalase from porcine kidney
CAS:9025-52-9
Purity:buffered aqueous glycerol solution, >=1.0 units/mg protein Package:5UN Remarks:T8778-5UN
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ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE manufacturers
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| | ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE Basic information |
| Product Name: | ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE | | Synonyms: | TREHALASE;ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE;EC 3.2.1.28;trehalase from porcine kidney;α,α-trehalose glucohydrolase;Native Porcine Trehalase;α,α-trehalase;Trehalase from porcine kidney buffered aqueous glycerol solution, >=1.0 units/mg protein | | CAS: | 9025-52-9 | | MF: | | | MW: | 0 | | EINECS: | 805-523-5 | | Product Categories: | | | Mol File: | Mol File | ![ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE Structure]() |
| | ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE Chemical Properties |
| density | 1.32-1.42g/cm3 at 20℃ | | storage temp. | -20°C | | form | buffered aqueous glycerol solution | | LogP | -1.3 at 20℃ and pH5.7-5.8 |
| | ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE Usage And Synthesis |
| Uses | Trehalase has been used:
- in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
- in a study to investigate growth arrest by trehalose-6-phosphate
- to quantify the intracellular trehalose in Lactobacillus casei cells
| | General Description | Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification. | | Biochem/physiol Actions | Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature. | | Purification Methods | This trehalase is purified by solubilising in Triton X-100 and sodium deoxycholate, and submitting to gel filtration, ion-exchange chromatography, conA-Sepharose chromatography, phenyl-Sepharose CL-4B hydrophobic interaction chromatography, Tris-Sepharose 6B affinity and hydrolyapatite chromatography. Activity is increased 3000-fold. [Yoneyama Arch Biochem Biophys 255 168 1987.] T4-RNA ligase (from bacteriophage-infected E.coli ) Mr 43,500, [EC 6.5.1.3 for RNA lyase]. This ligase is purified by differential centrifugation and separation on a Sephadex A-25 column, then through hydroxylapatite and DEAE-glycerol using Aff-Gel Blue to remove DNAase activity. (Greater than 90% of the protein in the enzyme preparation migrated as a single band on gradient polyacrylamide gels containing SDS during electrophoresis.) [McCoy et al. Biochim Biophys Acta 562 149 1979.] |
| | ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE Preparation Products And Raw materials |
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