流感病毒血凝素(HA)肽
中文名称 | 流感病毒血凝素(HA)肽 |
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中文同义词 | 流感病毒血凝素(HA)肽;HA多肽;人类流感血凝素多肽H-TYR-PRO-TYR-ASP-VAL-PRO-ASP-TYR-ALA-OH;流行性感冒血凝素 (HA) 肽 |
英文名称 | HA PEPTIDE |
英文同义词 | INFLUENZA HEMAGGLUTININ (HA) PEPTIDE;H-TYR-PRO-TYR-ASP-VAL-PRO-ASP-TYR-ALA-OH;HEMAGGLUTININ PRECURSOR (114-122) [INFLUENZA A VIRUS];HA1 FRAGMENT 99-107;H2N-YPYDVPDYA-OH;HA TAG PEPTIDE;TYR-PRO-TYR-ASP-VAL-PRO-ASP-TYR-ALA;YPYDVPDYA |
CAS号 | 92000-76-5 |
分子式 | C53H67N9O17 |
分子量 | 1102.17 |
EINECS号 | |
相关类别 | 细胞信号和神经生物学;多肽;细胞生物学;目录多肽;EpitopeTags;Peptide |
Mol文件 | 92000-76-5.mol |
结构式 |
流感病毒血凝素(HA)肽 性质
沸点 | 1556.4±65.0 °C(Predicted) |
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密度 | 1.414±0.06 g/cm3 (20 ºC 760 Torr) |
储存条件 | -20°C |
溶解度 | DMSO 中≥55.1 mg/mL;乙醇中≥100.4 mg/mL;水中≥46.2 mg/mL |
形态 | 结晶固体 |
酸度系数(pKa) | 3.38±0.10(Predicted) |
序列 | H-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-OH |
HA Peptide is a highly immunoreactive tag generally used for the separation of tagged proteins from cell culture supernatants and cell lysate under neutral pH conditions and thus are handy tools for coimmunoprecipitation but are also easily detected via western blot. HA Peptide is small and thus unlikely to interfere with the bioactivity and function of the fusion partner proteins. HA Peptide comes from human influenza hemagglutinin (HA) corresponding to amino acids 98–106 and is a strong immunoreactive epitope making it popular to isolate, purify, detect, and track the protein of interest. The recombinant HA-tagged proteins can be separated by highly specific anti-HA monoclonal antibody that is covalently immobilized on resin. The HA-tagged proteins can be eluted by mild elution approach with HA epitope at 1 mg/mL in TBS. On the other hand, three chemical elution options are available: 0.1 M glycine (pH 2-2.8), 3 M NaSCN, or 50 mM NaOH. The nucleotide sequences encoding an N-terminal HA Peptide in the mammalian expression vectors is an essential element for the T7 promoter-driven expression in E. coli even without trans-acting T7 RNAP. Research results suggest that HA Peptide is cleaved by caspase 3/7, and HA Peptide cleavage results in a total loss of immunoreactivity. Observations indicate that the use of HA to tag proteins and constructs to study cell death–related and apoptotic mechanisms can result in serious artifacts.
纯度(HPLC) ≥98.0%
醋酸根含量≤12.0%
水分含量≤8.0%
肽含量≥80.0%
内毒素≤50EU/mg
氨基酸组成分析≤±10%
安全信息
WGK Germany | 3 |
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提供商 | 语言 |
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英文
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更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
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2024/01/25 | HY-P0239 | TYR-PRO-TYR-ASP-VAL-PRO-ASP-TYR-ALA HA Peptide | 92000-76-5 | 1mg | 500元 |
2024/01/25 | HY-P0239 | TYR-PRO-TYR-ASP-VAL-PRO-ASP-TYR-ALA HA Peptide | 92000-76-5 | 5mg | 990元 |