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| | 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Basic information |
| Product Name: | 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE | | Synonyms: | TMB*2HCL*2H2O;3,3',5,5'-tetramethylbenzidine·2HCl·2H2O;3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%;3,3μ,5,5μ-Tetramethylbenzidine hydrate dihydrochloride;3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate,TMB dihydrochloride;TMB (hydrochloride hydrate);3,3',5,5'-tetraMethylbiphenyl-4,4'-diaMine dihydrochloride hydrate (1:2:);3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate | | CAS: | 207738-08-7 | | MF: | C16H23ClN2O | | MW: | 294.82 | | EINECS: | 688-511-0 | | Product Categories: | | | Mol File: | 207738-08-7.mol |  |
| | 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Chemical Properties |
| Melting point | >300°C | | storage temp. | 2-8°C | | solubility | Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml). | | form | Powder | | Sensitive | Moisture & Light Sensitive | | InChIKey | NYNRGZULARUZCC-UHFFFAOYSA-N | | CAS DataBase Reference | 207738-08-7 |
| Risk Statements | 22 | | Safety Statements | 22-24/25-25-24 | | WGK Germany | 3 | | TSCA | Yes | | HS Code | 29215900 |
| | 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Usage And Synthesis |
| Description | TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase. | | Chemical Properties | Off-white fluffy powder | | Uses | 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB). | | Uses | Convenient water-soluble form of TMB for determination of peroxidase. | | Uses | 3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine. | | General Description | 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes. |
| | 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE Preparation Products And Raw materials |
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