人参皂苷RK2
| 中文名称 | 人参皂苷RK2 |
|---|---|
| 中文同义词 | 人参皂苷RK1(RK1:RZ1=5:2) |
| 英文名称 | Ginsenoside RK2 |
| 英文同义词 | PAN 30;3beta,12beta-Dihydroxydammar-20(21),24-diene-3-O-beta-D-glucopyranosyl(1-2)-beta-D-glucopyranoside;Ginsenoside Rk1 (Rk1:Rz1=5:2);β-D-Glucopyranoside, (3β,12β)-12-hydroxydammara-20,24-dien-3-yl 2-O-β-D-glucopyranosyl-;Ginsenoside RK2 USP/EP/BP;3β,12β-dihydroxydammar-20(21),24-diene-3-O-β-D-glucopyranosyl(1->2)-β-D-glucopyranoside;(2S,3R,4S,5S,6R)-2-(((2R,3R,4S,5S,6R)-4,5-dihydroxy-2-(((3S,5R,8R,9R,10R,12R,13R,14R,17S)-12-hydroxy-4,4,8,10,14-pentamethyl-17-(6-methylhepta-1,5-dien-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol |
| CAS号 | 494753-69-4 |
| 分子式 | C42H70O12 |
| 分子量 | 767 |
| EINECS号 | |
| 相关类别 | 标准品;对照品;中药对照品;分析试剂标准品;对照品-中药对照品;标准品-对照品;标准品，对照品 |
| Mol文件 | 494753-69-4.mol |
| 结构式 |  |
人参皂苷RK2 性质
| 沸点 | 854.5±65.0 °C(Predicted) |
|---|---|
| 密度 | 1.27±0.1 g/cm3(Predicted) |
| 储存条件 | 2-8°C |
| 溶解度 | 溶于甲烷 |
| 形态 | 粉末 |
| 酸度系数(pKa) | 12.85±0.70(Predicted) |
Rk2一般通过水解人参皂苷二醇组获得，但水解成分复杂，不利于分离并准确鉴定结构。本文通过酶法水解高纯度的人参皂苷Rk2，水解产物通过高分辨质谱和核磁确证。
Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation.
RT-PCR
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells. |
Western Blot Analysis
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells |
Cell Viability Assay
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 48 hours |
| Result: | Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner. |
Cell Cycle Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced G0/G1 phase arrest. |
Apoptosis Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in MDA-MB-231 cells. |
药理药效:抗癌、提高免疫功能、调节神经系统。