IB-MECA
| 中文名称 | IB-MECA |
|---|---|
| 中文同义词 | N6-(3-碘苄基)腺苷-5'-N-甲基糖酰胺;腺苷A3受体激动剂(IB-MECA);化合物PICLIDENOSON;(2S,3S,4R,5R)-3,4-二羟基-5-(6-((3-碘苄基)氨基)-9H-嘌呤-9-基)-N-甲基四氢呋喃-2-甲酰胺;PICLIDENOSON IB-MECA; CF-101;IB-MECA,A 3激动剂 |
| 英文名称 | IB-MECA |
| 英文同义词 | 1-DEOXY-1-[6-[[(3-IODOPHENYL)METHYL]AMINO]-9H-PURIN-9-YL]-N-METHYL-BETA-D-RIBOFURANURON-AMIDE;Selepressin;RPR-113090;3-iodobenzyl-5'-N-methylcarboxamidoadenosine;IB-MECA;N6-(3-IODOBENZYL)ADO-5'N-METHYLURONAMIDE;n(6)-(3-iodobenzyl)-5’-n-methylcarboxamidoadenosine;IB-MECA SELECTIVE A3 ADENOSIN |
| CAS号 | 152918-18-8 |
| 分子式 | C18H19IN6O4 |
| 分子量 | 510.29 |
| EINECS号 | |
| 相关类别 | 细胞生物学试剂;医药原料 |
| Mol文件 | 152918-18-8.mol |
| 结构式 |  |
IB-MECA 性质
| 密度 | 1.98±0.1 g/cm3(Predicted) |
|---|---|
| 储存条件 | 2-8°C |
| 溶解度 | 热乙醇：>10 mg/mL |
| 酸度系数(pKa) | 12.72±0.70(Predicted) |
| 形态 | 固体 |
| 颜色 | 白色 |
| InChIKey | HUJXGQILHAUCCV-MOROJQBDSA-N |
A3AR
Piclidenoson is able to inhibit Forskolin (HY-15371)-stimulated cAMP levels with EC
50
s of 0.82 μM and 1.2 μM in OVCAR-3 cells and Caov-4 cells, respectively.
Piclidenoson (0.0001-100 μM; 48 hours) significantly reduces cell viability in a dose-dependent manner in human ovarian cancer cell lines, with IC
50
s of 32.14 μM and 45.37 μM for OVCAR-3 and Caov-4 cells, respectively.
Piclidenoson (0.001-100 μM; 48 hours) induces apoptosis in ovarian cancer cell line through the caspase pathway.
Piclidenoson induces apoptosis via the mitochondrial signaling pathway.
Cell Proliferation Assay
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 0.0001-100 μM |
| Incubation Time: | 48 hours |
| Result: | Resulted in a dose-dependent reduction in the cell viability. |
Apoptosis Analysis
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 0.1 μM, 1 μM, 10 μM, 50 μM, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Significant increased in the percentage of apoptosis in a concentration-dependent manner. |
Western Blot Analysis
| Cell Line: | OVCAR-3 cells, Caov-4 cells |
| Concentration: | 1 μM, 10 μM, 100 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased the expression of Bcl-2 was noticeably and increased the expression of Bax protein. |
Piclidenoson (105 μg/kg; i.p.) enhances survival of γ-irradiated mice.
| Animal Model: | B10CBAF1 male mice aged 3 months (average 30 g) |
| Dosage: | 105 μg/kg |
| Administration: | Intraperitoneal injection, 0.5 h after irradiation |
| Result: | Resulted in statistically significant increases of the mean survival time in comparison with the control irradiated mice. |
安全信息
| 安全说明 | 22-24/25 |
|---|---|
| WGK Germany | 3 |
| 提供商 | 语言 |
|---|---|
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英文
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