Company Name: |
Sigma-Aldrich
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Tel: |
021-61415566 800-8193336 |
Email: |
orderCN@merckgroup.com |
Products Intro: |
Product Name:Lectin from Glycine max (soybean) Purity:peroxidase conjugate, lyophilized powder Package:1MG Remarks:L2650-1MG
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Company Name: |
Riedel-de Haen AG
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Tel: |
800 558-9160 |
Email: |
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Products Intro: |
Product Name:Lectin from Glycine max (soybean)
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Company Name: |
SIGMA-RBI
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Tel: |
800 736 3690 (Orders) |
Email: |
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Products Intro: |
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| SBA, Soybean agglutinin Basic information |
Product Name: | SBA, Soybean agglutinin | Synonyms: | Lectin from Glycine max (soybean);SBA, Soybean agglutinin | CAS: | | MF: | | MW: | 0 | EINECS: | | Product Categories: | | Mol File: | Mol File | |
| SBA, Soybean agglutinin Chemical Properties |
| SBA, Soybean agglutinin Usage And Synthesis |
Uses | General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
| Biochem/physiol Actions | SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine. |
| SBA, Soybean agglutinin Preparation Products And Raw materials |
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