1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
生物活性靶点体外研究 用途与合成方法 MSDS 1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine价格(试剂级) 上下游产品信息
| 中文名称 | 1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine |
|---|---|
| 中文同义词 | 化合物SPP-86;SPP 86,RET抑制剂;1-异丙基-3-(苯基乙炔基)-1H-吡唑并[3,4-D]嘧啶-4-胺 |
| 英文名称 | 1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine |
| 英文同义词 | 1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine;SPP 86;1H-Pyrazolo[3,4-d]pyrimidin-4-amine, 1-(1-methylethyl)-3-(2-phenylethynyl)-;SPP86 >=98% (HPLC);Inhibitor,inhibit,SPP-86,SPP 86,SPP86,RET;1-Isopropyl-3-(phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine |
| CAS号 | 1357349-91-7 |
| 分子式 | C16H15N5 |
| 分子量 | 277.32 |
| EINECS号 | |
| 相关类别 | |
| Mol文件 | 1357349-91-7.mol |
| 结构式 | ![1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 结构式](CAS/20200331/GIF/1357349-91-7.gif) |
1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 性质
| 沸点 | 495.3±40.0 °C(Predicted) |
|---|---|
| 密度 | 1.23±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | 溶于二甲基亚砜 |
| 酸度系数(pKa) | 3.95±0.30(Predicted) |
SPP-86 是一种选择性的 RET 酪氨酸激酶的有效抑制剂，其 IC50 值为 8 nM。SPP-86 抑制 MCF7 细胞中 RET 诱导激活的 PI3K/Akt 和 MAPK 信号通路，也能抑制 RET 诱导的 ERα 磷酸化。
IC50: 8 nM (RET).
SPP86 (0-10 μM) inhibits MAPK signaling and proliferation in RET/PTC1 expressing TPC1 but not 8505C or C643 cells.
SPP86 (0-10 μM) inhibits RET- induced phosphatidylinositide 3-kinases (PI3K)/Akt and MAPK signaling and estrogen receptorα (ERα) phosphorylation in MCF7 cells.
Western Blot Analysis
| Cell Line: | Thyroid cancer derived cell lines expressing the RET/PTC1 rearrangement (TPC1), BRAF V600E (8505C) or RAS G13R (C643) mutations. |
| Concentration: | 0-10 μM. |
| Incubation Time: | 90 min. |
| Result: | Inhibited RET- induced ERK1/2 phosphorylation in thyroid cancer cell lines. |
Western Blot Analysis
| Cell Line: | MCF7 cells (human breast cancer). |
| Concentration: | 0-10 μM. |
| Incubation Time: | 30 min. |
| Result: | Inhibited RET- induced ERα phosphorylation and proliferation in MCF7 cells. |
安全信息
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/04/30 | HY-110193 | 1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine SPP-86 | 1357349-91-7 | 1mg | 480元 |
| 2024/04/30 | HY-110193 | 1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine SPP-86 | 1357349-91-7 | 5mg | 1200元 |