1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile
| 中文名称 | 1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile |
|---|---|
| 中文同义词 | 1-氨基-3-(对甲苯基)苯并[4,5]咪唑并[1,2-A]吡啶-2,4-二甲腈;化合物IFSP1 |
| 英文名称 | 1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile |
| 英文同义词 | 1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile;Pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile, 1-amino-3-(4-methylphenyl)-;iFSP1;SW620,Gpx4-knockout,FSP1,Ferroptosis,cancer,FSP1-knockout,iFSP1,iFSP-1,iFSP 1,cell,U-373,A549,RSL3,NCl-H1437,inhibit,Inhibitor |
| CAS号 | 150651-39-1 |
| 分子式 | C20H13N5 |
| 分子量 | 323.35 |
| EINECS号 | |
| 相关类别 | 对照品 |
| Mol文件 | 150651-39-1.mol |
| 结构式 | ![1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile 结构式](CAS/20180711/GIF/150651-39-1.gif) |
1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile 性质
| 密度 | 1.31±0.1 g/cm3(Predicted) |
|---|---|
| 储存条件 | Store at -20°C |
| 溶解度 | DMSO:30.0(最大浓度 mg/mL);92.78(最大浓度 mM) |
| 形态 | 结晶固体 |
| 酸度系数(pKa) | 2.90±0.50(Predicted) |
| Target | Value |
|
FSP1/AIFM2
(Cell-free assay) | 103 nM(EC50) |
iFSP1 (0.001-1 μM; 24 hours) inhibits the Gpx4-knockout cell growth as a dose-dependent manner, but does not inhibit the wild type cell growth. Treatment with the ferroptosis inhibitor Lip-1 protects GPX4- knockout cells from iFSP1-induced ferroptosis.iFSP1 (0.001-1 μM; 24 hours) is less efficient than genetic deletion of FSP1, whereas iFSP1 treatment in the FSP1-knockout background had no additive effect to RSL3-induced ferroptosis.iFSP1 (3 μM; 24 hours) treatment results in an obvious toxicity of RSL3 in a panel of genetically engineered (FSP1-knockout) human cancer cell lines.AIFM2: the flavoprotein apoptosis-inducing factor mitochondria-associated 2 is a previously unrecognized anti-ferroptotic gene. AIFM2, which is renamed ferroptosis suppressor protein 1 (FSP1)
Cell Proliferation Assay
| Cell Line: | Wild-type and Gpx4-knockout Pfa1 or HT 1080 cells overexpressing FSP1–HA |
| Concentration: | 0.001-1 μM |
| Incubation Time: | 24 hours |
| Result: | Was toxic to cells that depend solely (no GPX4 expression detectable) on FSP1 function. |
Cell Viability Assay
| Cell Line: | NCl-H1437, NCl-H1437 FSP1 KO, U-373, U-373 FSP1 KO, MDA-MB-436, MDA-MB-436 FSP1 KO, SW620, SW620 FSP1 KO, MDA-MB-435S, MDA-MB-435S FSP1 KO, A549 and A549 FSP1 KO |
| Concentration: | 3 μM |
| Incubation Time: | 24 hours |
| Result: | Sensitized a variety of human cancer cell lines to the ferroptosis inducer (1S,3R)-RSL3. |
安全信息
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/04/30 | HY-136057 | iFSP1 | 5 mg | 300元 | |
| 2024/04/30 | HY-136057 | 1-amino-3-(4-methylphenyl)pyrido[1,2-a]benzimidazole-2,4-dicarbonitrile iFSP1 | 150651-39-1 | 10mg | 480元 |