2,7-双(2-羧乙基)-5(6)-羧基荧光素
中文名称 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素 |
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中文同义词 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素;2,7-双-(羧乙基)-羧基-荧光素;5(OR 6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸;2',7'-二-(2-羧乙基)-5(6)-羧基荧光素;2`,7`-双(2-羧基乙基)-5(6)-羧基荧光素;PH荧光探针BCECF,酸;5(OR6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1CHEMICALBOOK(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸;2',7'-二-(2-羧乙基)-5(6)-羧基荧光素 |
英文名称 | 2`,7`-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein |
英文同义词 | BCECF;2',7'-bis(Carboxyethyl)-5(6)-Carboxyfluorescein(BCECF);2',7'-bis(Carboxyethyl)-5(6)-Carboxyfluorescein (B;2',7'-Bis(carboxyethyl)-4or5-carboxyfluorescein;2'',7''-Bis-(2-carboxyethyl)-4(5)-carboxyfluorescein;2'',7''-BIS(2-CARBOXYETHYL)-5(6)- CARBOXYFLUORESCEIN (BCECF);2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN;2'7'-BIS-(2-CARBOXYETHYL)-5-(AND-6) FLUORESCEIN |
CAS号 | 85138-49-4 |
分子式 | C27H20O11 |
分子量 | 520.44 |
EINECS号 | |
相关类别 | 生化试剂;荧光探针,标记,颗粒和染色 |
Mol文件 | 85138-49-4.mol |
结构式 |
2,7-双(2-羧乙基)-5(6)-羧基荧光素 性质
储存条件 | -20°C |
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溶解度 | DMF:5mg/mL; DMSO:15mg/mL; DMSO:PBS (pH 7.2) (1:2):0.25 mg/ml;乙醇:5mg/mL |
形态 | 结晶固体 |
BRN | 8375770 |
Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K + /H + ionophor nigericin does not inhibit hydrogenosomal loading with BCECF.The pH-sensitive fluorescent dyes to measure cytosolic pH. 1.Prepare a 2 to 20 mM stock solution of BCECF in DMSO. 2.Prepare a 5-50 µM BCECF dye-loading solution in buffer solutions (HHBS or PBS).3. Add 1000 µL/well (6-well plate),100 µL/well (96-well plate) or 25 µL/well (384-well plate) BCECF dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at E x /E m = 490/535 nm or 430/535 nm for ratio measurements.
安全信息
危险品标志 | Xn |
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危险类别码 | 20/21/22-36/37/38 |
安全说明 | 22-24/25-36-26 |
WGK Germany | 3 |
F | 8 |
提供商 | 语言 |
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英文
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更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
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2024/01/25 | HY-101882 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素 BCECF | 85138-49-4 | 1mg | 1600元 |