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Riedel-de Haen AG
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Purity:clone M3A5, ascites fluid
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SIGMA-RBI
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800 736 3690 (Orders) |
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VWR International
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800 932 5000 |
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solutions@vwr.com |
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| | ANTI-GOLGI BETA-COP PROTEIN Basic information |
| | ANTI-GOLGI BETA-COP PROTEIN Chemical Properties |
| storage temp. | -20°C | | biological source | mouse |
| Hazard Codes | Xn | | Risk Statements | 21/22 | | Safety Statements | 36/37 | | WGK Germany | 3 |
| | ANTI-GOLGI BETA-COP PROTEIN Usage And Synthesis |
| Uses | Monoclonal Anti-β-COP antibody produced in mouse is suitable for use as a primary antibody in immunoblot:
- analysis at a working dilution of 1:1000 using subcellular proteins from rat PC12 (pheochromocytoma) cells
- analysis of gradient fractions of cerebral microvessels to confirm the separation of plasma membrane lipid raft domains from intracellular membranous components
- detection of the Golgi marker protein β-COP in exosome-enriched extracellular microvesicles (eMV) preparations from untreated HeLa cells
It is suitable for immunostaining of β-coatomer, that is used as a intracellular, Golgi protein marker :
- to confirm that CD14 staining is localized to the cell surface of HAEC
- for examining the localization of Meltrin β in the Golgi apparatus and its vicinity in neurons prepared from developing dorsal root ganglia of mouse embryos
- in NB4 and NB4-LR1 cells to examine the colocalization of PKA regulatory subunits
- in CHO cells to examine colocalization of GFP-Rab24
It is suitable for use in cell-surface ELISA of human aortic endothelial cells (HAEC)
It is also suitable for western blot analysis at a working dilution of 1:1000 using a preparation of stacked Golgi membranes from rat liver, for indirect immunofluorescence at a working dilution of 1:80 using cultured Chinese hamster ovary (CHO) cells and for microarray. | | General Description | The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins. | | Biochem/physiol Actions | COPs (coatomer proteins) contain adaptin-like, complex (8) and are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. β-COP has a molar mass of 110kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles. |
| | ANTI-GOLGI BETA-COP PROTEIN Preparation Products And Raw materials |
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