黄羽扇豆魏特酮
| 中文名称 | 黄羽扇豆魏特酮 |
|---|---|
| 中文同义词 | 黄羽扇豆魏特酮;5,7-二羟基-3-(4-羟基苯基)-8-(3-甲基-2-丁烯-1-基)-4H-苯并吡喃-4-酮 |
| 英文名称 | 8-PRENYLGENISTEIN |
| 英文同义词 | 8-PRENYLGENISTEIN;5,7-Dihydroxy-3-(4-hydroxyphenyl)-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one;3-(4-Hydroxyphenyl)-5,7-dihydroxy-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one;4',5,7-Trihydroxy-8-isopentenylisoflavone;4',5,7-Trihydroxy-8-prenylisoflavone;8-Isopentenylgenistein;4H-1-Benzopyran-4-one, 5,7-dihydroxy-3-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-;Luncheonette |
| CAS号 | 104691-86-3 |
| 分子式 | C20H18O5 |
| 分子量 | 338.35 |
| EINECS号 | |
| 相关类别 | 黄酮;标准品;Miscellaneous Natural Products |
| Mol文件 | Mol File |
| 结构式 |  |
黄羽扇豆魏特酮 性质
| 熔点 | 133-135℃ |
|---|---|
| 沸点 | 583.5±50.0 °C(Predicted) |
| 密度 | 1.351±0.06 g/cm3(Predicted) |
| 储存条件 | 4°C, protect from light |
| 溶解度 | DMSO：100 mg/mL（295.55 mM；需要超声波） |
| 形态 | 粉末 |
| 酸度系数(pKa) | 6.62±0.20(Predicted) |
apoptosis
Lupiwighteone (2-100 μM; 72 hours) shows cytotoxicity towards various cell lines, especially on DU-145 cells and SGC-7901 cells with IC
50
s of 23.7 μM and 21 μM, respectively.
Lupiwighteone (20-60 μM; 48 hours) induces (cell cycle arrest in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces cells apoptosis.
Lupiwighteone (20-60 μM; 48 hours) decreases the cell cycle-related protein expressions in a dose-dependent manner in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces a dose-dependent increase in ROS production.
Lupiwighteone up-regulates of cytochrome c and caspase-3, and subsequent cleavage of PARP-1 and down-regulates of the p-Akt/Akt ratio and VEGF expression, suggests the activation of mitochondria-based intrinsic apoptosis in DU-145 cells.
Cell Viability Assay
| Cell Line: | DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero |
| Concentration: | 2 μM, 5 μM, 25 μM, 50 μM, 100 μM |
| Incubation Time: | 72 hours |
| Result: | Had inhibition on the survival of many cancer cell lines and HUVEC. |
Cell Cycle Analysis
| Cell Line: | DU-145 cells |
| Concentration: | 20 μM, 40 μM, 60 μM |
| Incubation Time: | 48 hours |
| Result: | Induced cell cycle arrest. |
Apoptosis Analysis
| Cell Line: | DU-145 cells |
| Concentration: | 20 μM, 40 μM, 60 μM |
| Incubation Time: | 48 hours |
| Result: | Induced apoptosis. |
Western Blot Analysis
| Cell Line: | DU-145 cells |
| Concentration: | 20 μM, 40 μM, 60 μM |
| Incubation Time: | 48 hours |
| Result: | Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner. |