N-[苄氧羰基]-L-色氨酰-L-ALPHA-谷氨酰-N-[(1S)-3-氟-1-(2-甲氧基-2-氧代乙基)-2-氧代丙基]-L-组胺酰胺甲基酯
| 中文名称 | N-[苄氧羰基]-L-色氨酰-L-ALPHA-谷氨酰-N-[(1S)-3-氟-1-(2-甲氧基-2-氧代乙基)-2-氧代丙基]-L-组胺酰胺甲基酯 |
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| 中文同义词 | N-[苄氧羰基]-L-色氨酰-L-ALPHA-谷氨酰-N-[(1S)-3-氟-1-(2-甲氧基-2-氧代乙基)-2-氧代丙基]-L-组胺酰胺甲基酯 |
| 英文名称 | CASPASE-1 INHIBITOR TFA SALT |
| 英文同义词 | CASPASE-1 INHIBITOR TFA SALT;Z-WEHD-FMK-TFA;Z-TRP-GLU(OME)-HIS-ASP(OME)-FMK-TFA;N-[(Phenylmethoxy)carbonyl]-L-tryptophyl-L-alpha-glutamyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-L-histidinamide methyl ester;Caspase-1/ICE Inhibitor Z-WEHD-FMK;Z-WE(OME)HD(OME)-FMK;Z-TRP-GLU(OME)-HIS-ASP(OME)-FMK;Z-TRP-GLU(OME)-HIS-ASP(OME)-FLUOROMETHYLKETONE;L-Histidinamide, N-[(phenylmethoxy)carbonyl]-L-tryptophyl-L-α-glutamyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-, methyl ester (9CI) |
| CAS号 | 210345-00-9 |
| 分子式 | C37H42FN7O10 |
| 分子量 | 763.77 |
| EINECS号 | |
| 相关类别 | 多肽;Apoptosis |
| Mol文件 | 210345-00-9.mol |
| 结构式 | ![N-[苄氧羰基]-L-色氨酰-L-ALPHA-谷氨酰-N-[(1S)-3-氟-1-(2-甲氧基-2-氧代乙基)-2-氧代丙基]-L-组胺酰胺甲基酯 结构式](CAS/GIF/210345-00-9.gif) |
N-[苄氧羰基]-L-色氨酰-L-ALPHA-谷氨酰-N-[(1S)-3-氟-1-(2-甲氧基-2-氧代乙基)-2-氧代丙基]-L-组胺酰胺甲基酯 性质
| 沸点 | 1129.2±65.0 °C(Predicted) |
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| 密度 | 1.349±0.06 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | 不溶于水； ≥26.32 mg/mL，乙醇溶液（需要超声波）； DMSO 中≥46.33 mg/mL |
| 形态 | 粉末 |
| 酸度系数(pKa) | 11.06±0.46(Predicted) |
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Caspase-1
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Z-WEHD-FMK (80 μM; 9 hours) elicits a near-complete blockage of C. trachomatis -induced cleavage of golgin-84 and increases GM130 expression in cells.Z-WEHD-FMK (30 min before being exposed to E. piscicida ) effectively inhibits 0909I E. piscicida induced ZF4 cells cytotoxicity and pyroptotic morphology. And in addition, it also inhibits the cytotoxicity induced by cytosolic LPS delivery.Z-WEHD-FMK (20 μM;18-24 hours following Cr 3+ ,Ni 2+ , and Co 2+ ) significantly induces a decrease of 76% to 86% in IL-1β release with 200 to 400 ppm Cr 3+ , it also induces a decrease of 35% to 45% with 48 ppm Ni 2+ or higher, Finally, this caspase-1 inhibitor induced a decrease with 6 ppm Co 2+ , down to a level below the detection threshold, and a decrease of 40% to 48% with 12 to 24 ppm Co 2+ in bone marrow-derived macrophages (BMDM).
Western Blot Analysis
| Cell Line: | C. trachomatis - or mock-infected HeLa cells |
| Concentration: | 80 μM |
| Incubation Time: | 9 hours |
| Result: | Increased golgin-84 and GM130 expression. |
Cell Viability Assay
| Cell Line: | Mycoplasma free-ZF4 cells |
| Concentration: | |
| Incubation Time: | 30 min before being exposed to E. piscicida |
| Result: | Inhibited ZF4 cells cytotoxicity and pyroptotic morphology. |