5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩
| 中文名称 | 5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩 |
|---|---|
| 中文同义词 | 5-溴-2-(4-氟苯基)-3-(4-甲基磺酰基苯基)噻吩;化合物 T15181;DUP 697,环氧合酶2(COX-2)抑制剂 |
| 英文名称 | DUP-697 |
| 英文同义词 | DUP-697;5-BROMO-2-(4-FLUOROPHENYL)-3-(4-(METHYLSULFONYL)PHENYL)THIOPHENE;BFMeT;DuP-697 Assay Reagent;Thiophene, 5-bromo-2-(4-fluorophenyl)-3-[4-(methylsulfonyl)phenyl]-;DuP697,DuP 697 |
| CAS号 | 88149-94-4 |
| 分子式 | C17H12BrFO2S2 |
| 分子量 | 411.31 |
| EINECS号 | |
| 相关类别 | 医药中间体;Lipid signaling;API intermediates |
| Mol文件 | 88149-94-4.mol |
| 结构式 |  |
5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩 性质
| 储存条件 | 2-8°C |
|---|---|
| 溶解度 | DMF：54 mg/ml； DMF:PBS (pH 7.2) (1:1)：0.5 mg/ml； DMSO：15 mg/ml；乙醇：7 mg/ml |
| 形态 | 白色至类白色固体。 |
|
hCOX-2 10 nM (IC 50 ) |
hCOX-1 800 nM (IC 50 ) |
DuP-697 (0-100 nM; 24 hours; HT29 cells) treatment shows antiproliferative with an IC
50
value of 42.8 nM.
DuP-697 (25-100 nM; 72 hours; HT29 cells) treatment causes concentration dependent apoptosis in HT29 cells. The percentage of UR (apoptosis portion) area increases gradually according to the concentration of DuP-697 from 7% in control group to 52% in 100 nM DuP-697.
DuP-697 in 100, 10 and 1 nM concentrations cause antiangiogenic effect. Antiangiogenic scores of DuP-697 are 1.2, 0.8 and 0.5, respectively.
Cell Proliferation Assay
| Cell Line: | HT29 cells |
| Concentration: | 0 nM, 12.5 nM, 25 nM, 50 nM, 100 nM |
| Incubation Time: | 24 hours |
| Result: | Exhibited decreasing CI values in a concentration dependent manner. Showed statistically significant cytotoxic effect. |
Apoptosis Analysis
| Cell Line: | HT29 cells |
| Concentration: | 25 nM, 50 nM, 100 nM |
| Incubation Time: | 72 hours |
| Result: | Caused concentration dependent apoptosis in HT29 cells. |
DuP-697 is a potent inhibitor of paw swelling in nonestablished and established adjuvant arthritis in rats (ED
50
= 0.03 and 0.18 mg/kg/day, respectively). DuP-697 has no effect on phenylquinone writhing in rats (ED
50
greater than 100 mg/kg), but is analgetic against inflammation-related pain in the Randall-Selitto assay (ED
50
= 3.5 mg/kg) and is a very potent antipyretic agent (ED
50
= 0.05 mg/kg). DuP-697 (5 mg/kg i.v.) does not alter renal blood flow or the renal vascular response to angiotensin II in furosemide-pretreated, volume-depleted rats.
DuP-697 is a moderate inhibitor of bull seminal vesicle prostaglandin (PG) synthesis (IC
50
of 24 μM) and a potent inhibitor of rat brain PG synthesis (IC
50
of 4.5 μM) but was ineffective against rat kidney PG synthesis (IC
50
of 75 μM).