荧光标记淀粉样蛋白肽(1-40)
| 中文名称 | 荧光标记淀粉样蛋白肽(1-40) |
|---|---|
| 中文同义词 | 荧光标记淀粉样蛋白肽(1-40) |
| 英文名称 | 5-FAM-Amyloid β-Protein (1-40) |
| 英文同义词 | 5-FAM-Amyloid β-Protein (1-40);β-Amyloid (1-40), FAM-labeled;5-FAM-Amyloid β-Protein (1-40) trifluoroacetate salt;5-FAM-Amyloid β-Protein (1-40) |
| CAS号 | 1678416-08-4 |
| 分子式 | |
| 分子量 | 0 |
| EINECS号 | |
| 相关类别 | |
| Mol文件 | Mol File |
| 结构式 |
荧光标记淀粉样蛋白肽(1-40) 性质
λ ex = 492 nm; λ em = 518 nm
Apical-to-basolateral exchange across endothelial monolayer of fluorescent Aβ42 (FAM-labeled Human β-Amyloid (1-42) and Fluor ™ 488-labeled β-Amyloid (1-42)) or scramble Aβ42 ( FAM-labeled scrambled β-Amyloid (1-42) ) (1-100 μM) is monitored in transendothelial electrical resistance (TEER) during cell monolayer’s formation over time (over 120 min in presence or absence of Aβ24). Human β-Amyloid (1-24) (1 μM) results in H-Aβ42 retention, thus reducing its efflux through the BBB and therefore preventing an efficient mechanism of Aβ42 clearance.The in vitro BBB model is used to investigate the passage of FAM-labeled or 488-conjugated H-Aβ42 across the endothelial cell monolayer. The addition of fluorescently labeled H-Aβ42 (FAM-labeled Human β-Amyloid (1-42) or Fluor ™ 488-labeled β-Amyloid (1-42)) to the apical side of cell inserts results in effective BBB crossing, FAM-labeled scrambled β-Amyloid (1-42) is not efficiently transcytosed.