中文名称

血浆/血清外泌体纯化和RNA小提试剂盒（提取试剂盒）

英文名字

Plasma/Serum Exosome Purification and RNA Isolation Mini Kit

供应商

Norgen Biotek

产品货号

NGB-58300

背景资料

血浆/血清外泌体纯化和RNA小提试剂盒（提取试剂盒）产品的背景资料详情查看Norgen Biotek品牌提供的产品说明书

产品描述

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 µL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

产品特点

Purification and enrichment of intact plasma/serum exosomes for functional studies; Bind and elute all RNA irrespective of size or GC content, without bias; Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA; Versatile plasma/serum input volume (50 µL - 1 mL); No phenol extractions, Proteinase K treatment, nor carrier RNA required; No time-consuming ultracentrifugation, filtration nor special syringes required; No precipitation reagents, nor overnight incubation required; Compatible with plasma/serum from any species; Pure exosomes are purified and are free-from any other RNA-binding proteins.

保存建议

所有缓冲液应密封保存，并在室温（15-25°C）下保存长达2年。

其他

Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。 目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。