Description
DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2.
Catalog No.
RGK60001
Host species
mouse
Tested applications
CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IP, SPR, IF
Species reactivity
General
Form
liquid
Storage buffer
0.01M PBS, pH 7.4.
Concentration
0.74 mg/ml
Purity
>95%
Clonality
Monoclonal
Isotype
IgG2a, kappa
Applications
CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IP, SPR, IF
Purification
Protein A or G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Expression system
Mammalian cells
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.
Reconstitution
Reconstitute in sterile water for a stock solution.
Alternative Names
DNA-RNA Hybrid
Note
For research use only.
Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids. PMID: 33830170
Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody. PMID: 32681514
High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing. PMID: 31053798
RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. PMID: 29742442
The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences. PMID: 28594954
Structural basis of R-loop recognition by the S9.6 monoclonal antibody. PMID: 35347133
Best practices for the visualization, mapping, and manipulation of R-loops. PMID: 33411340
Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287
The Affinity of the S9.6 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast. PMID: 29289567
The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S9.6. PMID: 23784994
Modifications and interactions at the R-loop. PMID: 32961406
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. PMID: 24896180
R-Loop Analysis by Dot-Blot. PMID: 33554969
Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead. PMID: 34324345
New insight into the biology of R-loops. PMID: 32516653
Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR. PMID: 33371452
Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation. PMID: 32105733
Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids. PMID: 25890199
Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. PMID: 34495394
关键字： DNA-RNA,antibody,抗体;s9.6,经典抗体;
武汉佰乐博生物（Biolab Reagents）始创于 2021 年，核心创始团队由五位毕业于武汉大学、华中科技大学、华中农业大学等知名高校的生命科学领域顶尖研究者组成。团队成员均深耕行业二十载，积淀了深厚的产品开发实力与成熟的技术应用经验，为企业发展筑牢核心技术根基。
佰乐博生物是国家高新技术企业、国家科技型中小企业，技术实力获权威认可；同时通过 ISO9001 质量管理体系、ISO13485 医疗器械质量管理体系双认证，以标准化、规范化的品控体系，筑牢产品品质基石。公司依托全球领先的技术平台，专注于高品质生物试剂的全球化开发与整合，与法国 AntibodySystem 品牌达成深度战略合作，系其在亚洲的独家授权合作伙伴以及生工生物KleanAB品牌的代理商。目前已构建起近 60000 种生命科学试剂的丰富产品矩阵，核心产品涵盖蛋白、抗体、试剂盒三大品类，重点覆盖肿瘤、神经系统等疾病研究热门领域，可全维度满足各类科研实验的多元需求。
自成立以来，佰乐博生物始终秉持初心，致力于为广大生命科学科研工作者、生物医药工业客户提供专业、全面、可靠的试剂产品与技术支持，以优质科研工具赋能科研探索，助力生命科学研究领域实现创新发展与深度突破。