Rabbit Anti-Insulin receptor subunit beta Polyclonal Antibody
DATASHEET
Host:Rabbit
Target Protein:Insulin receptor subunit beta
IR:Immunogen Range:801-900/1382
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3643
Swiss Prot:P06213
Source:KLH conjugated synthetic peptide derived from human Insulin receptor subunit beta:801-900/1382
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism.
Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
Size:50ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
Flow-Cyt(1μg/Test)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
仅供科研使用。不能用于人和动物治疗等其它临床诊断用！
关键字： Insulin receptor sub;CD220;CD220 antigen;HHF5;INSR;nsulin receptor;Insulin receptor sub;INSR_HUMAN;
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