中文名:蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8
英文名:Endoproteinase Glu-C from Staphylococcus aureus V8
中文别名:V8蛋白酶
纯度:≥500 units/mg dry weight
货号:P128655
包装:1mg、5mg
Cas号:66676-43-5
存储温度:2-8°C储存
产品介绍:
Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping .
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