中文名：6-羧基四甲基罗丹明
英文名：6-Carboxytetramethylrhodamine
纯度：适用于荧光分析，≥90%
货号：C115506
Cas号：91809-67-5
存储温度：避光,-20°C储存,干燥
运输条件：超低温运输
产品介绍：
6-羧基四甲基罗丹明（6-TAMRA），是用来建立荧光DNA探针和标记蛋白质/肽。相应的琥珀酰亚胺酯是一个单一的异构体荧光团,在DNA自动测序时最为常用。
1).Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO).
2).Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations.
3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations.
4).Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.
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关键字： 6-Carboxytetramethylrhodamine, BioReagent, suitable for fluorescence, >=90% (HPLC) ; MFCD01073638 ; SY078324 ; 2-[3,6-Bis(dimethylamino)xanthylium-9-yl]-4-carboxybenzoate
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