基本信息
产品名称:Recombinant AMPK alpha 1 Antibody
规格或纯度:重组, ExactAb™, 已验证, 见COA
应用:WB, IF/ICC, IHC
特异性:PRKAA1
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:A synthetic peptide derived from human AMPK alpha 1 (AA 500-559).
阳性对照:WB: Raw264.7, MCF7, C6, K562, Hep G2, NIH/3T3 and HeLa cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:重组抗体
Format:Whole IgG
亚型:Rabbit IgG
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂:0.02% Sodium azide
浓度:见COA
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联:Unconjugated
图片

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Western Blot: Recombinant AMPK alpha 1 Antibody (Ab088655)
Recombinant AMPK alpha 1 Antibody (Ab088655) - Western Blot
All lanes: Recombinant AMPK alpha 1 Antibody (Ab088655) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 66 kDa
Exposure time: 5.0 s

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IF/ICC: Recombinant AMPK alpha 1 Antibody (Ab088655)
Recombinant AMPK alpha 1 Antibody (Ab088655) - IF/ICC
Immunocytochemistry analysis of AMPK alpha 1 (green) in HeLa cells using Recombinant AMPK alpha 1 Antibody (Ab088655), and cells were counterstained with DAPI (blue).

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IHC: Recombinant AMPK alpha 1 Antibody (Ab088655)
Recombinant AMPK alpha 1 Antibody (Ab088655) - IHC
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Recombinant AMPK alpha 1 Antibody (Ab088655). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab088655.html



关键字: 5 AMP activated protein kinase alpha 1catalytic subunit antibody ; 5 AMP activated protein kinase catalytic alpha 1 chain antibody ; 5' AMP activated protein kinase catalytic subunit alpha 1 antibody ; 5'-AMP-activated protein kinase catalytic subunit alp
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